Community Characteristics Analysis of Eukaryotic Microplankton via ITS Gene Metabarcoding Based on Environmental DNA in Lower Reaches of Qiantang River, China

Aiju Zhang; Jun Wang; Yabin Hao; Shanshi Xiao; Wei Luo; Ganxiang Wang; Zhiming Zhou

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Community Characteristics Analysis of Eukaryotic Microplankton via ITS Gene Metabarcoding Based on Environmental DNA in Lower Reaches of Qiantang River, China

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Eukaryotic microplankton plays an important role in water biotic community and in maintaining the stability of water ecosystems. Environmental DNA metabarcoding provides the opportunity to integrate traditional and emerging approaches to discover more new species, and develop molecular biotic indices that can be more rapidly, frequently, and robustly used in water quality assessments. In order to examine assemblages of eukaryotic microplankton in lower reaches of Qiantang River, ITS gene metabarcoding technology based on environmental DNA was carried out. As a result, various species of phytoplankton, fungi and zooplankton were annotated on. More phylum, classes and specieses of eukaryotic phytoplankton and zooplankton were found after compared communities taxa based on metabarcoding with that obtained from morphological examination. Nevertheless, Chlorophyceae was the most common assemblage both identified by using these two methods, also Mesocyclops leuckarti and Acanthocyclops bicuspidatus were both found to be the dominant species of Cyclopoida in the river. Additionally, the reads proportions of phytoplankton and zooplankton at the three freshwater sampling sites (Tonglu, Fuyang and Wenyan) decreased as temperature drop. Meanwhile, twenty classes of fungi were annotated on, of which the community characteristic was first researched in the river. There were significant spatial differences in values of Chao1 index for eukaryotic microplankton. Cluster analysis and Non-metric multidimensional scaling ordination further confirmed that the community composition of eukaryotic microplankton at class level for Jiashao-September sample had the most dissimilarity with the others.

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《动物科学期刊（英文）》 |2021年第2期|P.105-124|共20页
作者
Aiju Zhang; Jun Wang; Yabin Hao; Shanshi Xiao; Wei Luo; Ganxiang Wang; Zhiming Zhou;
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作者单位

Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province Zhejiang Research Center of East China Sea Fishery Research Institute Zhejiang Institute of Freshwater Fisheries Huzhou China;

Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province Zhejiang Research Center of East China Sea Fishery Research Institute Zhejiang Institute of Freshwater Fisheries Huzhou China;

Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province Zhejiang Research Center of East China Sea Fishery Research Institute Zhejiang Institute of Freshwater Fisheries Huzhou China;

Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province Zhejiang Research Center of East China Sea Fishery Research Institute Zhejiang Institute of Freshwater Fisheries Huzhou China;

Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province Zhejiang Research Center of East China Sea Fishery Research Institute Zhejiang Institute of Freshwater Fisheries Huzhou China;

Pinghu Fisheries Technology Promotion Center Pinghu China;

Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture Key Laboratory of Freshwater Aquaculture Genetic and Breeding of Zhejiang Province Zhejiang Research Center of East China Sea Fishery Research Institute Zhejiang Institute of Freshwater Fisheries Huzhou China;

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原文格式 PDF
正文语种 chi
中图分类水生生物学;
关键词
Microplankton; eDNA; Metabarcoding; ITS; Diversity; Qiantang River;

机译：Microplankton;EDNA;Metabarcoding;它;多样性;钱塘河;

Community Characteristics Analysis of Eukaryotic Microplankton via ITS Gene Metabarcoding Based on Environmental DNA in Lower Reaches of Qiantang River, China

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