首页> 中文期刊> 《肿瘤学研究(英文)》 >Stromal and Tumor Glioma-derived Cells with Similar Characteristics Have Differences in α-Smooth Muscle Actin Expression and Localization

Stromal and Tumor Glioma-derived Cells with Similar Characteristics Have Differences in α-Smooth Muscle Actin Expression and Localization

         

摘要

Gliomas are solid brain tumors composed of tumor cells and recruited heterogenic stromal components.The study of the interactions between the perivascular niche and its surrounding cells is of great value in unraveling mechanisms of drug resistance in malignant gliomas.In this study,we isolated the stromal diploid cell population from oligodendroglioma and a mixed population of tumor aneuploid and stromal diploid cells from astrocytoma specimens.The stromal cells expressed neural stem/progenitor and mesenchymal markers showing the same discordant phenotype that is typical for glioma cells.Moreover,some of the stromal cells expressed CD133.For the first time,we demonstrated that this type of stromal cells had the typical myofibroblastic phenotype as theα-SMA+cells formedα-SMA fibers and exhibited the specific function to deposit extracellular matrix(ECM)proteins at least in vitro.Immunofluorescent analysis showed diffuse or focalα-SMA staining in the cytoplasm of the astrocytoma-derived,A172,T98G,and U251MG glioma cells.We could suggest thatα-SMA may be one of the main molecules,bearing protective functions.Possible mechanisms and consequences ofα-SMA disruptions in gliomas are discussed.

著录项

  • 来源
    《肿瘤学研究(英文)》 |2021年第2期|P.8-21|共14页
  • 作者单位

    Institute of Cytology Russian Academy of Sciences St.Petersburg 194064 Russia;

    Institute of Cytology Russian Academy of Sciences St.Petersburg 194064 Russia;

    Institute of Cytology Russian Academy of Sciences St.Petersburg 194064 Russia;

    Institute of Cytology Russian Academy of Sciences St.Petersburg 194064 Russia;

    Institute of Cytology Russian Academy of Sciences St.Petersburg 194064 Russia;

    Almazov National Medical Research Centre St.Petersburg 197341 Russia;

    Peter the Great Saint-Petersburg Polytechnic University St.Petersburg 195251 Russia;

    Institute of Cytology Russian Academy of Sciences St.Petersburg 194064 Russia;

    Almazov National Medical Research Centre St.Petersburg 197341 Russia;

    Institute of Cytology Russian Academy of Sciences St.Petersburg 194064 RussiaAlmazov National Medical Research Centre St.Petersburg 197341 RussiaScientific Technological University«Sirius» Sochi 354340 Russia;

    Institute of Cytology Russian Academy of Sciences St.Petersburg 194064 Russia;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 肿瘤学;
  • 关键词

    Oligodendroglioma; Astrocytoma; Primary cell cultures; Tumor microenvironment; Myofibroblasts; Extracellular matrix;

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