CCL2 Enhances the Viability of Human Chorionic Trophoblast Cell Line HTR-8/SVneo Cells by Inhibiting Interleukin-24 Expression

Jun Shao; Yin-Yan He; Da-Jin Li; Ming-Qing Li

首页> 中文期刊> 《生殖与发育医学：英文版》 >CCL2 Enhances the Viability of Human Chorionic Trophoblast Cell Line HTR-8/SVneo Cells by Inhibiting Interleukin-24 Expression

CCL2 Enhances the Viability of Human Chorionic Trophoblast Cell Line HTR-8/SVneo Cells by Inhibiting Interleukin-24 Expression

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相关主题

摘要

Background:To investigate the regulatory effect of interleukin-24(IL-24)on cell viability of human chorionic trophoblast cell line(HTR-8/SVneo cells).Methods:Immunohistochemical staining was used to detect the expression of IL-24 and its receptors IL-20R1,IL-20R2,and IL-22R1 in villus tissue at early normal pregnancy.The effect of thymic stromal lymphopoietin(TSLP)and chemokine CCL2 on the expression of IL-24 in human chorionic trophoblast cell line HTR-8/SVneo cells was analyzed by In-cell Western.In addition,the effect of recombinant human IL-24(rhIL-24)and CCL2 on the viability of HTR-8/SVneo cells was analyzed by MTT assay.Results:IL-24 and its receptors showed a strong positive staining in trophoblasts at early normal pregnancy.Compared with control group,expression of IL-24 in HTR-8/SVneo cells was significantly inhibited after in vitro stimulation of recombinant human CCL2 protein(rhCCL2)(P<0.001).The viability of HTR-8/SVneo cells was significantly decreased after treatment with rhIL-24(P<0.001).In contrast,anti-IL-24 neutralizing antibody significantly enhanced the viability of HTR-8/SVneo cells(P<0.01).In addition,rhCCL2(100μg/L);enhanced the viability of HTR-8/SVneo cells(P<0.01)in vitro,but this effect was inhibited by treatment with rhIL-24.Conclusions:CCL2 enhances the viability of human trophoblast cell line HTR-8/SVneo cells in vitro by inhibiting the secretion of IL-24,which may be beneficial to blastocyst implantation and placental development.

著录项

来源
《生殖与发育医学：英文版》 |2017年第4期|P.198-203|共6页
作者
Jun Shao; Yin-Yan He; Da-Jin Li; Ming-Qing Li;
展开▼
作者单位

Laboratory for Reproductive Immunology Hospital and Institute of Obstetrics and Gynecology Fudan University Shanghai 200011 China;

Department of Obstetrics and Gynecology Shanghai General Hospital Shanghai Jiao Tong University Shanghai 200080 China;

Laboratory for Reproductive Immunology Hospital and Institute of Obstetrics and Gynecology Fudan University Shanghai 200011 ChinaKey Laboratory of Reproduction Regulation of NPFPC Shanghai 200032 China;

Laboratory for Reproductive Immunology Hospital and Institute of Obstetrics and Gynecology Fudan University Shanghai 200011 ChinaKey Laboratory of Reproduction Regulation of NPFPC Shanghai 200032 China;

展开▼
原文格式 PDF
正文语种 chi
中图分类肿瘤学;
关键词
CCL2; Cell Viability; Chorionic Trophoblasts; HTR-8/SVneo Cells; Interleukin-24;

CCL2 Enhances the Viability of Human Chorionic Trophoblast Cell Line HTR-8/SVneo Cells by Inhibiting Interleukin-24 Expression

摘要

著录项

相关主题

期刊订阅