首页> 中文期刊> 《食品科学与人类健康(英文)》 >A synthetic biological secondary metabolite,Lycogen^TM,produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy

A synthetic biological secondary metabolite,Lycogen^TM,produced and extracted from Rhodobacter sphaeroides WL-APD911 in an optimizatioal scale-up strategy

         

摘要

The optimization of fermentation medium is important for synthetic biological secondary metabolite productions.The effect of rotation speed,inoculum amount,and medium supplements on the cell growth and Lycogen^TM secretion of photobacterium Rhodobacter sphaeroides WL-APD911 was evaluated.The results reveal that a higher rotational speed exhibit a higher cell density,and the increasing in the amount of inoculum amount show a slight augment on the growth of R.sphaeroides WL-APD911.In the case of nitrogen sources adding,Lycogen^TM production was achieved with a 0.5 mM l-lysine supplementation.Moreover,the attention of Tween 80 presented a tremendous increase in the secondary metabolite.Response surface methodology(RSM)exhibited the optimization of medium supplements for Lycogen^TM invention is accomplished at molasses concentration of 10 g/L,yeast extract concentration of 40 g/L,0.3%Tween 80 and NaCl concentration of 5 g/L,respectively.Further,the batch fermentation is carried out in both 5 L and 20 L fermentors to study the scale-up process factors to be adopted.At a 20 L fermentor,Lycogen^TM yields under the optimal culture condition are over 2 times than in the shake flask.The present results provide the Lycogen^TM optimal culture mediums,scale-up procedures and efficient extractions from R.sphaeroides WL-APD911.

著录项

  • 来源
    《食品科学与人类健康(英文)》 |2017年第4期|195-201|共7页
  • 作者单位

    Research and Development Department;

    Chia-Yi Steel;

    Tainan 717;

    Taiwan;

    China;

    College of Oceanology and Food Science;

    Quanzhou Normal University;

    Quanzhou 362000;

    China;

    Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae;

    Quanzhou 362000;

    China;

    Division of Plastic Surgery;

    Department of Surgery;

    Kaohsiung Medical University Hospital;

    Kaohsiung Medical University;

    Kaohsiung 807;

    Taiwan;

    China;

    Graduate Institute of Medicine;

    College of Medicine;

    Kaohsiung Medical University;

    Kaohsiung 807;

    Taiwan;

    China;

    Center for Stem Cell Research;

    Kaohsiung Medical University;

    Kaohsiung 807;

    Taiwan;

    China;

    Department of Surgery;

    Faculty of Medicine;

    College of Medicine;

    Kaohsiung Medical University;

    Kaohsiung 807;

    Taiwan;

    China;

    Orthopaedic Research Center;

    Kaohsiung Medical University;

    Kaohsiung 807;

    Taiwan;

    China;

    Doctoral Degree Program in Marine Biotechnology;

    National Sun Yat-sen University and Academia Sinica;

    Kaohsiung 804;

    Taiwan;

    China;

    Marine Biomedical Laboratory and Center for Translational Biopharmaceuticals;

    Department of Marine Biotechnology and Resources;

    National Sun Yat-sen University;

    Kaohsiung 804;

    Taiwan;

    China;

    Graduate Institute of Biomedical Engineering;

    National Chung Hsing University;

    Taichung 402;

    Taiwan;

    China;

    Department and Graduate Institute of Aquaculture;

    National Kaohsiung Marine University;

    Kaohsiung 811;

    Taiwan;

    China;

    Asia-Pacific Biotech Developing;

    Inc.Kaohsiung 803;

    Taiwan;

    China;

    Department of Medical Research;

    China Medical University Hospital;

    China Medical University;

    Taichung 404;

    Taiwan;

    China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 有机化学;
  • 关键词

    Rhodobacter sphaeroides WL-APD911; Lycogen^TM; Response surface methodology(RSM); Ferementation;

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