Determination of Telomere Length by the Quantitative Fluorescence iin Situ/iHybridization (Q-FISH) Method

Junko Aida; Naotaka Izumiyama-Shimomura; Ken-ichi Nakamura; Naoshi Ishikawa; Masanori Terai; Yoko Matsuda; Shinsuke Aida; Tomio Arai; Kaiyo Takubo

首页> 中文期刊> 《美国分析化学（英文）》 >Determination of Telomere Length by the Quantitative Fluorescence iin Situ/iHybridization (Q-FISH) Method

Determination of Telomere Length by the Quantitative Fluorescence iin Situ/iHybridization (Q-FISH) Method

开具论文收录证明 >>

期刊封面封底目录下载 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Telomeres are nucleoprotein complexes located at the ends of eukaryotic chromosomes and are shorten with aging or various causes. Shortened telomere plays an important role for chromosomal instability in carcinogenesis, or a number of diseases in relation to aging. FISH using peptide-nucleic acid (PNA) probe is suitable for analyzing telomeres, because telomere is a part of DNA molecule and localized in chromosomal end in loop shape structure (T-loop). We introduce our telomere analysis for tissue sections and cultured cells in this paper. On the tissue sections, we analyze telomere length with telomere intensity to centromere intensity ratio (TCR), centromere as an inner control. In the cultured cells, we analyze telomere lengths on each chromosome with karyotyping. Those Q-FISH methods by PNA probe are an accurate and reliable tool for research on telomere lengths in various conditions in biology.

著录项

来源
《美国分析化学（英文）》 |2014年第12期|775-783|共9页
作者
Junko Aida; Naotaka Izumiyama-Shimomura; Ken-ichi Nakamura; Naoshi Ishikawa; Masanori Terai; Yoko Matsuda; Shinsuke Aida; Tomio Arai; Kaiyo Takubo;
展开▼
作者单位

Department of Pathology;

International University of Health and Welfare Mita Hospital;

Tokyo;

Japan;

Department of Pathology;

Tokyo Metropolitan Geriatric Hospital;

Tokyo;

Japan;

Research Team for Geriatric Pathology;

Tokyo Metropolitan Institute of Gerontology;

Tokyo;

Japan;

展开▼
原文格式 PDF
正文语种 chi
中图分类肿瘤学;
关键词
Telomere; Telomere; Length; Q-FISH; Tissue; Section; Chromosome;

相似文献

中文文献
外文文献

Determination of Telomere Length by the Quantitative Fluorescence iin Situ/iHybridization (Q-FISH) Method

摘要

著录项

相似文献

相关主题

期刊订阅