Establishment of Cytokines Quantitative PCR Methods and Its Application in Anaphylaxis Study of Houttuynia Cordata Injection

Xiangqi Zeng; Fengjiao Sun; Xiaolu Cheng; Wen Zeng; Li Li

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Establishment of Cytokines Quantitative PCR Methods and Its Application in Anaphylaxis Study of Houttuynia Cordata Injection

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We design specific primers, according to the four kinds of important macaque cytokines, based on macaque β-action genes, then extract total RNA in peripheral blood mononuclear cells of macaques, and trascriptase cDNA reversely after establishing real-time quantitative PCR, and test the relative expression amount of several kinds of important cytokines during the hypersensitive process. The testing method of quantitative PCR in this experiment is highly sensitive, all cytokines are up to 102 copies/μL and highly reproducible, the intraassay and interassay coefficients of variation are less than 6%. The method applied to the sensitive reaction experiment on cytokines induced by houttuynia cordata turns out that the cytokines relative expression of houttuynia cordata distillate with low dose group and high dose group is significantly higher than that of saline group (P > 0.01). Meanwhile, significantly increased IL-4, IL-10 and IL-13 and decreased IFN-γ are found in both group A and group B after houttuynia injection (P > 0.05).

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《生物科学与医学（英文）》 |2015年第7期|1-8|共8页
作者
Xiangqi Zeng; Fengjiao Sun; Xiaolu Cheng; Wen Zeng; Li Li;
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作者单位

Leshan Vocational and Technical College;

Leshan;

China;

Key Laboratory of Animal Disease and Human Health of Sichuan Province;

College of Veterinary Medicine;

Sichuan Agricultural University;

Ya’an;

China;

Sichuan Primed Shines Biotech Co.;

Ltd.;

National Experimental Macaque Reproduce Laboratory;

Ya’an;

China;

Leshan Hospital of Traditional Chinese Medicine;

Leshan;

China;

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原文格式 PDF
正文语种 chi
中图分类肿瘤学;
关键词
Cytokines; Quantitative; PCR; Anaphylaxis; Houttuynia; Cordata; Injection;

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Establishment of Cytokines Quantitative PCR Methods and Its Application in Anaphylaxis Study of Houttuynia Cordata Injection

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