首页> 中文期刊> 《世界肾病学杂志:英文版》 >Kinin B_2 receptor does not exert renoprotective effects on mice with glycerol-induced rhabdomyolysis

Kinin B_2 receptor does not exert renoprotective effects on mice with glycerol-induced rhabdomyolysis

         

摘要

AIM:To investigate a potential protective role of the kinin B2receptor in a glycerol-induced rhabdomyolysis mouse model.METHODS:We separated 28 C57Bl/6 male mice into4 groups:untreated WT animals,untreated B2knockout mice,glycerol-treated WT and glycerol-treated B2knockout mice.Glycerol-treated animals received one intramuscular injections of glycerol solution(50%v/v,7 m L/kg).After 48 h,urine and blood samples were collected to measure creatinine and urea levels.Additionally,kidney samples were extracted for histological evaluation,and the m RNA expression levels of kinin B1 and B2 receptors and inflammatory mediators were measured by real-time polymerase chain reaction.RESULTS:Serum creatinine and urea levels showed differences between untreated wild-type and glyceroltreated wild-type mice(0.66±0.04 vs 2.61±0.53mg/d L,P30 times,31.34±8.9)in kidney.Animals injected with glycerol had a higher degree of tubular injury than untreated animals.Wild-type and knockout mice treated with glycerol intramuscularly present kidney injury,with impairment in renal function.However,B2knockout mice treated with glycerol did not show a different phenotype regarding kidney injury markers,when compared to the wild-type glycerol-treated group.CONCLUSION:We conclude that the kinin B2receptordoes not have a protective role in renal injury.

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