Chicken manure was used as inoculum. Functional bacteria which deoxidize phosphorus and produce PH_3 were enriched in an anaerobic continuous stirred reactor. The removal efficiency of phosphorus was investigated under nitrate/sulfate/different car-bon source and different nitrogen source. The relations of phosphine production and nitrate/total phosphorus/ammonia removal were studied. The results show that: (1) Suitable addition of SO_4~(2-) -S and NO_3~--N is 26 mg·L~(-1) and no. Inorganic substance of oxidation state and phosphorus in the water compete for [H] under anaerobic conditions and lead to decrease phosphorus removal efficiency. (2)The best carbon source is Glucose (1 000 mg·L~(-1)). Cellulose is not suitable for carbon source. The best nitrogen source is pep-tone(500 mg·L~(-1)).The reductive sugar and organic nitrogen source in the water promote the generation of phosphine. (3)The pH value is controlled in the range of 6.5～7.0 and the most suitable growth temperature is about 35 ℃. (4) The removal efficiency of ammo-nia is improved with that of phosphorus. Under anaerobic conditions nitrogen (ammonia is removed) and phosphorus (phosphorus is reduced into phosphine) can be eliminated at the same time. Phosphorus is removed by anaerobic bacteria deoxidizing phosphorus into phosphine and ammonia is decreased by the generation of nitrogen and protein.%采用鸡粪污泥为种泥,在厌氧混合连续流反应装置内进行厌氧还原磷产生磷化氧功能菌的富集,进行硝酸盐、硫酸盐、不同碳源和氮源条件下厌氧除磷效率的研究,并考察磷化氧的生成与硝酸、总磷、氨氮去除的关系.结果表明,(1)SO_4~(2-)-S适宜的投加量为26 mg·L~(-1),不投加NO_3~--N.水中含有氧化态的无机物在厌氧条件下与磷争夺[H]导致厌氧除磷的效率下降.(2)合适的碳源为葡萄糖1 000 mg·L~(-1),纤维素不适合作为碳源,合适的氮源为蛋白胨500 mg·L~(-1),水中含有的还原糖和有机氮源促进磷化氧的生成.(3)pH值控制在6.5～7.0的范围,最适宜的生长温度在35℃左右.(4)氨氮的去除率随着总磷的去除率而增加,在厌氧条件下可达到同时脱氮除磷的效果.磷的去除由厌氧除磷菌还原磷生成磷化氧完成,氨氮由生成氮气或生成蛋白质来去除.
展开▼
