In order to understand the promotion mechanism of iron and nickel in the coal bed methane,F420,hydrogenase,and cellulase were taken as the objects to research the effect of iron,nickel and combination of them on coal bed methane.Results showed that:1) The activity of F420 in the contrast samples were higer than that in the blank;2) the relationship between the activity of hydrogenase,cellulase and the concentration of single ion could explain the phenomenon of low promotion and high inhibition;the ability of Fe2+ to activate the enzyme was stronger than Ni2+;3)according to the time curve of activity concentration changes with hydrogenase and cellulase,the activities of hydrogenase,cellulase in the best combination of iron and nickel were higher than that of them even in the optimized concentration of single ion.%为了解铁和镍对煤制生物甲烷发酵的促进机制,选择F420、氢化酶、纤维素酶3种辅因子为对象,研究铁镍及两者组合对煤发酵产甲烷的影响.结果表明:1)对照样比空白样的辅酶F420活性要高;2)氢化酶、纤维素酶活与单因子浓度关系表明,存在低促高抑效应,且Fe2激活酶活性的能力要比Ni2强;3)两种酶活性随时间变化曲线表明,最佳离子组合时酶活都高于单离子最佳浓度.
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