利用实验室分离获得铜绿假单胞菌GF31(Pseudomonas aeruginosa GF31,简称菌株GF31),采用气相色谱/质谱联用(GC/MS)分析技术,开展菌株GF31在实际土壤环境中对氯氰菊酯的降解特性和降解产物研究,并进行了模拟田间实验.结果表明,在土壤中菌株GF31降解氯氰菊酯的主要产物为二氯菊酸和间苯氧基苯甲酸;土壤质地、土壤处理、外加营养源等因素都会影响氯氰菊酯降解速率和产物分布.在有机质贫乏的土壤中菌株GF31仍具有良好降解效率,2种降解产物浓度在降解过程中均存在先升高后降低的规律,说明存在二次降解.在模拟田间实验中该菌株能有效降解土壤中质量浓度为80 mg/kg的氯氰菊酯,10d的降解率达69％以上.由此可见,该菌株在氯氰菊酯污染土壤的生物修复中有一定应用潜力.%A strain of Pseudomonas aeruginosa GF31 was isolated and preserved in the laboratory. The isolated GF31 was employed to degrade the cypermethrin in soil and the simulated field experiments were further carried out. The degradation characteristic and metabolites was analyzed by the methods of gas chromatography mass spectrome-try. Results showed that dichloroving acid and 3-phenoxybenzoic acid (3-PBA) were the two primary metabolites for the cypermethrin degradation when using strain GF31; the degradation rate and metabolites distribution could be influenced by soil quality, soil treatment and the addition of nutrient amendments. The degradation rate could be kept at a high level for the soil with a low content of organic matter. It was observed that the concentration of two metabolites increased at first and then decreased during the degradation process, which indicated further degradation of these metabolites. In the simulated field experiment, GF31 could effectively degrade the 80 mg/kg of cypermethrin in the soil and given the degradation rate of above 69% after 10 d. In a word, strain GF31 is an effectively cypermethrin-de-grading bacterium which can be applied to the treatment of cypermethrin-contaminated soil.
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