目的:基于网络药理学,初步探讨"胆酸-猪去氧胆酸"的功效物质基础和配伍机制.方法:通过数据库检索获得胆酸和猪去氧胆酸的化学成分、作用靶点及相关疾病等信息,构建"药物-成分-靶标-疾病"网络,进行基因本体(gene ontology,GO)分类富集分析、基于京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)的通路富集分析,研究"胆酸-猪去氧胆酸"的作用机制.结果:"药物-成分-靶标-疾病"网络包含2个药物,2种活性成分,相应靶标6个,相关疾病8种.关键靶点涉及盐皮质激素受体、肝X受体-α氧化型胆固醇受体和肝X受体-β氧化型胆固醇受体等,关键疾病涉及动脉粥样硬化等.GO富集分析包含GO条目87个,其中生物过程相关条目68个,主要涉及胞饮负调控、三酰甘油生物合成正调控等方面;分子功能相关条目19个,主要涉及类固醇激素受体活性、配体依赖核受体活性等方面.KEGG通路富集分析包含KEGG通路2条,分别为胰岛素抵抗和甲状腺激素信号通路.结论:本研究结果初步验证了"胆酸-猪去氧胆酸"的基本药理作用及其机制,并为进一步深入揭示其作用机制奠定了良好基础.%OBJECTIVE: To carry out preliminary investigation on the material bases of efficacy and mechanism of making prescriptions of "cholic acid-hyodeoxycholic acid" based on the network pharmacology .METHODS: Data of chemical compositions , action targets and related diseases of cholic acid and hyodeoxycholic acid were retrieved to construct"drug-composition-target-disease"network and conduct classified enrichment analysis of gene ontology ( GO) and pathway enrichment analysis based on Kyoto encyclopedia of genes and genomes ( KEGG ) , so as to investigate the functional mechanism of "cholic acid-hyodeoxycholic acid".RESULTS:The "drug-composition-target-disease" network contained 2 kinds of drugs, 2 kinds of active compositions, 6 related targets and 8 kinds of related diseases.Key target spots involved mineralocorticoid receptors , liver X receptor-αoxysterols and liver X receptor βoxysterols , key diseases included atherosclerosis and so on .GO enrichment analysis contained 87 GO items , of which 68 involved biological process , mainly included negative regulation of pinocytosis and positive regulation of triacylglycerol biosynthesis;19 items involved molecular function, mainly included steroid hormone receptor activity and ligand-dependent nuclear receptor activity .KEGG pathway enrichment analysis contained 2 pathways, respectively were signaling pathways of insulin resistance and thyroid hormones . CONCLUSIONS:This study has preliminarily verified the basic pharmacological actions and the mechanism of "cholic acid-hyodeoxycholic acid", and laid foundations for further revealing the functional mechanism .
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