Objective To compare three different methods used in vitamin A determination in soft capsule and to provide reference for the assay of vitamin A and its preparations. Method UV, NP-HPLC and RP-HPLC were chosen in this research. Cyclohexane was used as solvent in the triple wavelength method of UV; Column of NP- HPLC was filled with Hypersil SiO2 (250×4. 6mm.5μm) and the mobile phase consisted of n-hexane and isopropyl alcohol (99. 8 : 0. 2); Column of RP- HPLC was packed with C1H(150×4. 6mm,5μm) and the mobile phase was methanol-water(97 : 3). Results The content of vitamin A determined by UV was 95. 5% , RSD = 1. 3%(n = 5); 112. 5%,RSD= 3.2%(n = 5)by NP-HPLC and 102.3%, RSD= 1. 0%(n = 5) by RP-HPLC. Conclusion Both UV and RP-HPI.C are convenient measurements for vitamin As NP-HPLC shows advantages in the test of impurities, while its stability is relatively poor.%目的:比较测定维生素A含量的3种不同方法,为维生素A及其制剂的质量研究提供参考.方法:分别采用紫外(UV)三点校正法与正、反相高效液相色谱法(HPLC)测定维生素A含量.UV三点校正法以环己烷为溶剂,按照三点校正法测定;NP-HPLC采用Hypersil Si()2柱(250 mm×4.6 mm,5 μm),流动相为正己烷异丙醇(99.8:0.2);RP-HPLC采用C18柱(150 mm×4.6 mm,5 μm),流动相为甲醇-水(97:3).结果:UV三点校正法测得VA含量为95.5％,RSD=1.3％(n=5);NP-HPLC为112.5％,RSD=3.2％(n=5);RP-HPLC为102.3％,RSD=1.0％(n=5).结论:UV三点校正法和RP-HPLC法操作简单易行,NP-HPLC法能较好地控制杂质,但系统稳定性相对较差.
展开▼
