The changes of γ-aminobutyric acid (GABA),GAD activity,the soluble protein and other nitrogen compounds in germinating process of tartary buckwheat were investigated.The result indicated that with the increase of germinating time,the GABA content of germinating tartary buckwheat reach maximum at the fourth day,and the GAD activity of cotyledon and embryo of germinated tartary buckwheat increased first and then decreased.The content of soluble protein decreased,the free amino acids content increased first and then decreased,and the content of whole amino acid increased.Besides methionine,several essential amino acids are increased such as isoleucine and valine.The glutamic acid increased,and all this changes could be used to improve GABA accumulation in germinated tartary buckwheat.%研究了苦荞发芽过程中γ-氨基丁酸(GABA)、谷氨酸脱羧酶(GAD)活力及可溶性蛋白、氨基酸等含氮物含量的变化.结果表明:随着发芽时间的延长,GABA含量在发芽4d时达到最高,子叶和胚中GAD活力均呈先升后降趋势.可溶性蛋白呈下降趋势,游离氨基酸含量先升高后降低,总氨基酸含量不断升高.除蛋氨酸外,异亮氨酸及缬氨酸等几种人体必需氨基酸均呈现上升趋势,谷氨酸(Glu)含量升高,这些变化均有利于发芽苦荞中GABA的富集.
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