以纳米材料为载体,以双酚A适配体及互补链为生物识别单元,建立了基于功能化磁纳米粒子及金纳米粒子的辣根过氧化物酶酶联增敏检测平台,可实现对食品基质中双酚A的快速前处理和痕量检测,该方法将可与双酚A特异性结合的适配体偶联于磁性纳米材料上,再与适配体互补链及辣根过氧化物酶(HRP)修饰的金纳米粒子进行竞争性结合,通过HRP对底物的催化水解反应引起450 nm处特征峰的变化来定量检测双酚A.结果表明:该检测体系在0～100 ng/mL时具有线性关系(R2 =0.978 4),检测限低至0.5 pg/mL,具有较好的实用性,为更好地检测食品基质中的双酚A提供了有力的技术支持.%A horseradish peroxidase enzyme-sensitized detection platform based on functionalized magnetic nanoparticles and gold nanoparticles was established with the nanomaterials as the carrier and the bisphenol A aptamer and the complementary strand as the biometric recognition unit.The method about rapid pretreatment and trace detection of bisphenol A in food matrix was established.The BPA aptamer binding to the magnetic nanomaterials could compete with the complementary strands of the aptamer and the horn root peroxidase (HRP) modified gold nanoparticles.The effect of HRP on the catalytic hydrolysis of the substrate resulted in the change of characteristic peaks at 450 nm,helping to quantitatively detect bisphenol A,which had the advantages of good selectivity,high sensitivity and good specificity.The results showed that this detection system had a linear relationship (R2 =0.978 4) in the concentration range of 0～ 100 ng/mL,and the limit of detection was as low as 0.5 pg/mL.This work provideed strong technical support for detection of bisphenol A in future.
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