为了获得高效通用的水果果肉DNA提取方法，本研究以11种水果为试验材料，从前处理方法、裂解液成分、异丙醇沉淀时间等方面对CTAB法进行了改进，并比较了行业标准法、试剂盒法、改良CTAB法3种方法的提取效果，发现改良CTAB法提取的水果果肉DNA浓度都在46.25μg/mL以上，纯度都在1.8左右，且能扩增出137 bp的18SrDNA条带，从而确定了高效通用的水果果肉DNA提取方法：改良CTAB法。%In order to obtain an efficient and general DNA extraction method from fresh fruit ,eleven kinds of fruit were taken as experimental material,the CTAB method was developed from several aspects,such as the pre-treatment methods,the composition of lysis buffer,the time of Isopropyl alcohol precipitation and so on. Furthermore,the three methods were compared on their extraction efficiency which included industry standard method,DNA extraction kit method and modified CTAB method. The results showed that modified CTAB method was the most suitable for the DNA extraction from fresh fruit ,the concentration of isolated genomic DNAs was in more than 46.25μg/mL,the value of OD260/OD280 was close to 1.8,and the isolated genomic DNAs also am-plified the 137 bp size banding of endogenous 18SrDNA .
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