Researching on the conditions of Trichoderma viride immobilized with self-flocculation ,so we could get high cellulase activity. First,we measured the effects of time,volume,pH,glucose and peptone concentra-tions on cellulase activity of self-flocculating immobilized Trichoderma viride. Then we used the orthogonal test design to obtain optimized combinations. The times ,volumes and pH had a bell shaped curve effect on cellulase activity of immobilized Trichoderma viride with self-flocculating. The glucoses had small effect on cellulase ac-tivity,but the peptone had great effect on cellulase activity. The best optimal conditions of self-flocculation of immobilized Trichoderma viride were 6 d of time,70 mL of volume,6 of pH,2.25%of glucoses and 1.50%of peptones. Under the optimized conditions,the cellulase activity could reach 3.55 U after Trichoderma viride for cultivating of 72 h.%探究绿色木霉自絮凝固定化条件，以便提高其分泌纤维素酶的能力。首先测定固定化时间、体积、pH、葡萄糖与蛋白胨浓度对自絮凝固定化绿色木霉分泌纤维素酶的影响，然后用正交试验设计进行优化组合。时间、体积和pH对自絮凝绿色木霉分泌纤维素酶的影响均呈钟形曲线；葡萄糖对自絮凝绿色木霉分泌纤维素酶影响不大；蛋白胨对自絮凝绿色木霉分泌纤维素酶影响较大。绿色木霉自絮凝固定化最优条件是：时间为6 d，pH为6，体积为70 mL，葡萄糖为2.25%，蛋白胨为1.50%。按此条件培养自絮凝固定化的绿色木霉72 h，分泌的纤维素酶活性为3.55 U。
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