The purpose of this study was to extract and purificate polysaccharide from the Pleurotus ferulae Lenzi , the basic physical and chemical properties of polysaccharide were obtained, and build a solid foundation at the further polysaccharide from the Pleurotus ferulae Lenzi exploration in the structure , relationship between structure and activity in theory. Pleurotus ferulae polysaccharide was prepared by ethanol precipitation of water extract of the Pleurotus Ferulae,that preliminarily extracted by ethanol,followed by removal of proteins,dialysis against water,precipitaion with alcohol and further purification by chromatography on DEAE-cellulose column and sephadex G-100 column. The thin layer chromatography indicated that polysaccharides from pleurotus ferulae were consisted of four monosaccharides, namely glucose, mannose, galactose and fructose. Basic on infrared and electrophoresis to analysis the mushroom ferulae polysaccharide fractions of structure , and it proved that Pleurotus ferulae polysaccharide was a kind of more pure polysaccharides. Finally, through the analysis by the AFM proved that Pleurotus ferulae polysaccharide molecule had highly branched structure, even between the sugar chain formed ring structure.%通过对新疆阿魏菇多糖进行提纯及分析，了解其基本理化性质，在理论上为深入探究阿魏菇多糖的结构以及构效活性奠定了基础。阿魏菇的热水提取液经乙醇沉淀、去蛋白、逆向流水透析、DEAE-Cellulose和Sephadex G-100柱层析得纯品阿魏菇多糖。薄层色谱分析表明阿魏菇总多糖是由半乳糖，葡萄糖，果糖，甘露糖组成。以红外和电泳为基础，分析了阿魏菇多糖组分的结构，表明其是一种较纯的多糖，原子力显微镜分析表明阿魏菇多糖分子具有高度分枝结构。
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