In this paper, the extraction and separation of the total flavones from pitaya flower were studied. Based on single-factor experiment, the extraction process conditions were optimized by using orthogonal test. Results showed that the optimal extraction conditions were as follows:ethanol concentration 70%, ratio of raw material to ethanol solution 1∶40(g/mL), extraction temperature 75℃and extraction time 2 h. Under the above conditions, the content of total flavones from dry pitaya flower was 0.747%. The average recovery rate and RSD were 99.51%and 2.06%respectively (n=5).%研究火龙果花中黄酮类化合物的分离提取和测定方法。在单因素实验的基础上，利用正交实验对分离提取条件进行优化。结果表明，最佳分离提取条件为：乙醇浓度为70%，固液比为1∶40（g/mL），提取温度为75℃，提取时间为2 h；以芦丁为标准品，采用AlCl3显色-分光光度法测得火龙果花中总黄酮含量为0.747%，样品平均回收率为99.51%， RSD值为2.06%（n=5）。
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