The antibacterial active substance produced by the marine antagonistic bacteria DX-10 were purified by the method of ethanol precipitation.The acute toxicity test , the test of micronucleus of polyehromatic erythrocytphilie of mouse and mouse sperm abnormality test were performed to examine the toxicological safety properties of the antibacterial active substance.Inhibitory activities of the antibacterial active substance against Vibrio parahaemolyticus, Staphylococcus aureus and Salmonella enteritidis sprayed in the surface of cod fish fillet were tested by viable bacteria counts counts. The results showed that performed the oral LD 50 in mice was greater than 50 g/kg, and two terms of hereditary toxicity were all negative; the growth of Vibrio parahaemolyticus, Staphylococcus aureus and Salmonella enteritidis was significantly inhibited by the antibacterial active substance, and the inhibition was in a dose-dependent manner. The antibacterial active substance produced by the marine antagonistic bacteria was a kind of safe and non-toxic substance with good antimicrobial activity and could be developed as a new natural biological preservative applied in the storage and processing of aquatic products.%采用乙醇沉淀法纯化海洋源拮抗菌DX-10发酵液中的抗菌活性物质，通过急性毒理试验、小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸变试验对该活性物质进行毒理学安全性评价；同时选取鳕鱼片通过表面喷洒的方式污染副溶血性弧菌、金黄色葡萄球菌和沙门氏菌，采用平板活菌计数法测定不同浓度抗菌活性物质对副溶血性弧菌、金黄色葡萄球菌、沙门氏菌的的抑菌效果。结果显示，小鼠急性经口LD50大于50 g/kg，小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸形试验结果均为阴性；拮抗菌DX-10产抗菌活性物质对副溶血性弧菌、金黄色葡萄球菌、沙门氏菌的生长有抑制作用，其抑制率呈现浓度依赖性。由此可见，拮抗菌DX-10产抗菌活性物质具有较高抗菌活性且安全无毒，可以将其开发为新型的天然生物防腐剂，应用在水产品企业生产加工储藏过程中。
展开▼
