[目的]香樟齿喙象是近年来发现的一种危害香樟的单食性蛀干害虫,为探索其系统发育地位和遗传进化关系,本研究分别扩增香樟齿喙象的mtDNA COI、18S rDNA V4、V7区和28S rDNA D2区序列.[方法]采用邻接法(neighbour-joining)和极大似然法(maximum likelihood)构建不同序列的系统发育树,分析香樟齿喙象与象甲科Cur-culionidae其它种类同源序列的碱基组成及系统进化特点,从分子水平探讨香樟齿喙象的分类地位.[结果]表明:COI与rDNA均显示香樟齿喙象与同科同族的魔喙象亚科Molytinae、树皮象族Hylobiini种类遗传距离最为相近,虽然无法比对到同属下的种类,但支持香樟齿喙象归为魔喙象亚科树皮象族的观点.[结论]通过比较发现,28S rD-NA序列分析结果印证了香樟齿喙象目前的分类地位,本研究为香樟齿喙象在分子水平上的鉴定提供了理论依据.%[Objective]To analyze the phylogenetic position and genetic evolution of Pagiophloeus tsushimanus. [Method]Four DNA sequence fragments:cytochrome oxidase subunitⅠ(COI)gene of mitochondrial DNA (mtD-NA),V4 and V7 region of 18S ribosomal DNA (18S rDNA)and D2 region of 28S ribosomal DNA (28S rDNA) were amplified.Maximum likelihood (ML)and neighbor jointing (NJ)methods were used to build up phylogenetic trees of P.tsushimanus and related species in Curculionidae,so as to study the classification status of P.tsushima-nus.[Result]Analysis on COI and rDNA suggested that P.tsushimanus belongs to Hylobiini of Molytinae.[Con-clusion]According to the analysis,the results of 28S rDNA support the morphological classification of P.tsushima-nus.
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