采用酶学分析法，检测野生与养殖条件下大鳍鳠（Mystus macropterus）前、中、后肠刷状缘膜（BBM）上蔗糖酶、麦芽糖酶、碱性磷酸酶、γ－谷氨酰转肽酶和 Na＋－K＋－ATP 酶活性。结果显示：（1）Na＋－K＋－ATP 酶在两种条件下大鳍鳠各肠段刷状缘膜活性大小不存在显著性差异，且 Na＋－K＋－ATP 酶的富集系数均大于1.0；（2）蔗糖酶、麦芽糖酶、γ－谷氨酰转肽酶和碱性磷酸酶在两种条件下大鳍鳠各肠道刷状缘膜上分布规律相同，其中，肠段刷状缘膜蔗糖酶、麦芽糖酶和碱性磷酸酶比活力在各肠段分布均为前肠＞中肠＞后肠，而γ－谷氨酰转肽酶比活力分布为中肠＞后肠＞前肠；（3）同种酶在不同条件下大鳍鳠同肠段的活性均为养殖大鳍鳠大于野生大鳍鳠，仅碱性磷酸酶比活力在野生大鳍鳠中肠略大于养殖大鳍鳠中肠。%The activities of five enzymes, sucrase, maltase, alkaline phosphatase, γ-glutamyltranspeptidase ( γ-GT) and Na+-K+-ATP of the enterocytes brush border membrane (BBM) of wild and farmed Mystus macropterus was investiga-ted by means of enzymes analyses .The results showed that: (1) The activity of Na+-K+-ATP in wild and that in farmed M.macropterus was not significant different from each other in all regions of the intestine , and both of the enrichment factors of Na+-K+-ATP were higher than 1.0; (2) The distribution characteristics of sucrase , maltase, alkaline phosphatase and γ-glutamyltranspeptidase (γ-GT) of the enterocytes brush border membrane (BBM) were the same, as well as, the activities of sucrase, maltase and alkaline phosphatase were foregut >midgut >hindgut, meanwhile activity of γ-glutamyl-transpeptidase (γ-GT) was midgut >hindgut >foregut.(3)The data rules of activities of the same enzymes in different re -gions of the intestine in these two fishes were that activities of the farmed M.macropterus′s enzymes were higher than the wild M.macropterus′s, except that the wild M.macropterus′s alkaline phosphatase activity in the midgut was higher than farmed M.macropterus′s.
展开▼
