首页> 中文期刊> 《淡水渔业》 >养殖鲈鲤肠道优势菌群组成及来源分析

养殖鲈鲤肠道优势菌群组成及来源分析

         

摘要

采用免培养16S rDNA的PCR-DGGE技术,对单一饲料养殖鲈鲤(Percoypris pingi pingi (Tchang))幼鱼(体重为13.61±1.86 g)肠道菌群(包括肠道壁、肠道内容物)、养殖水体、饲料等菌群结构进行了比较分析.结果显示:人工养殖的鲈鲤幼鱼肠道内容物菌群与肠道壁的菌群存在一定的相似性,肠道内容物菌群比肠道壁菌群丰富.中后肠肠道内容物菌群种类高于前肠,前中后肠道壁菌群无明显差别.鲈鲤幼鱼肠道菌群的来源主要是饲料,肠道内容物与饲料菌群相似性最高可达51.6%,肠道壁与饲料菌群相似性最高可达43.0%,因此在饲料中添加微生态制剂,来提高鲈鲤营养、抗病能力等是可行的.%The comparative analysis of bacterial community structure in aquaculture water, feed and in the intestinal canal of Percoypris pingi pingi larvae (body weight 13.61 ± 1.86 g) , including intestinal contents and intestinal wall, was conducted, based on 16S rDNA PCR-DGGE figure using a culture independent approach. The results showed that the bacterial community displayed a certain similarity between intestinal contents and intestinal wall of the fish under artificial rearing conditions. The bacterial community of intestinal contents was richer than that of intestinal wall. The bacterial communities of intestinal contents in midgut and hindgut were larger than that in foregut, but for intestinal wall there were no significant differences in bacterial communities among the fore - , mid - and hindgut. The main source of gastrointestinal micro-biota for the fish was feed. The bacterial communities of intestinal contents and intestinal wall had a greater relevance to the feed, with the values up to 51. 6% and 43. 0% , respectively. Therefore, it may be viable to add microbial ecological agents in the feed to improve the ability of disease resistance and nutrition intake for the fish.

著录项

  • 来源
    《淡水渔业》 |2011年第3期|29-33|共5页
  • 作者单位

    淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,西南大学生命科学学院,重庆400715;

    淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,西南大学生命科学学院,重庆400715;

    淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,西南大学生命科学学院,重庆400715;

    淡水鱼类资源与生殖发育教育部重点实验室,水产科学重庆市市级重点实验室,西南大学生命科学学院,重庆400715;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 水产微生物学;
  • 关键词

    鲈鲤(Percoypris pingi pingi(Tchang)); 肠道菌群; DGGE; 16S rDNA; 聚类分析;

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