Objective:To approach the practicability of the AllgloTM probe for the typing of IL-1 promoter . Methods: The genotyping for IL-1β promoter SNP was performed by the real-time Q-PCR with AllgloTM probe. We designed PCR primers and AllgloTM probe so as to detect the SNP site 3954C>T of IL-1β promoter,and optimized the concentration of primers and anneal temperature in accordance with PCR amplification efficiency and production specificity. Results: We successfully established the AllgloTM probe real-time Q-PCR detecting system, and in this two-step asymmetric PCR method, the primer density and the annealing temperature on the approximation PCR instrument were 0.4μM,58.9℃,respectively.We got the same genotyping by the system and sequenced in 100 healthy people detecting the site 3954C>T of IL-1β gene. Conclusion:The use of AllgloTM probe provides an attractive alternative for genotyping and detection of IL-1β gene SNP,which deserves to spread for its high-flux, low cost and convention.%目的：探讨AllgloTM探针检测IL-1单核苷酸多态性的实用性。方法：采用AllgloTM 探针实时荧光PCR对IL-1β基因SNP进行基因分型。设计其3954C>T位点AllgloTM探针序列和PCR扩增引物，按照PCR产物特异性和扩增效率，对退火温度、引物浓度等条件进行优化。结果：结果显示AllgloTM探针实时荧光PCR检测体系最适引物浓度为0.4μM，退化温度为58.9℃两步法PCR。在同一条件体系下，检测100例健康体检者IL-1β基因3954C>T位点，并进行基因分型，测序验证其分型结果。结论：AllgloTM探针实时荧光PCR检测IL-1β基因SNP是一种值得推广的高通量、低成本、常规化的基因分型方法。
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