目的 探讨葛根芩连汤对高脂饲料饲喂诱导的 NASH大鼠肝脏、小肠中炎症小体NLRP3相关因子的影响.方法 以高脂饮食建立大鼠NASH模型,并于造模同时进行药物干预;实验第8周末留取肝脏、小肠组织,Western blot及RT-PCR法检测炎症小体NLRP3相关因子蛋白及mRNA水平.结果 肝脏炎症小体NLRP3中:与空白组相比,模型组P10 mRNA及蛋白均有升高趋势,药物干预后其蛋白表达降低趋势明显(P<0.05).小肠炎症小体NLRP3中:与空白组相比,模型组P10蛋白表达升高,药物干预后表达下降(P<0.05).结论 高脂饲料饲喂的大鼠,小肠中炎症活化趋势强,炎症小体NLRP3中激活因素与稳定因素的平衡被打破,以炎症小体激活因素活化为主;肝脏中炎症小体亦有活化,但较易形成"高位"新的平衡;葛根芩连汤可减低激活因素活化程度、提高稳定因素活化程度,利于炎症小体内部平衡的恢复.%Objective To explore the effects of Gegen Qinlian decoction on NLRP3 in experimental non-alcoholic steatohepatitis (NASH). Methods 36 rats were randomly divided into 6 groups:the chow group,the model group,the high-dose Gegen Qinlian decoction group, the middle-dose Gegen Qinlian decoction group, the low-dose Gegen Qinlian decoction group and glutamine group. Treatments with saline, Gegen Qinlian decoctions and glutamine were given as the model establishment began. Liver tissue and small intestine tissue were harvested after 8 weeks treatment and NLRP3 was detected by Western-blot and RT-PCR. Results In liver, compared with chow group, the protein and mRNA levels of P10 in model group were increased. Gegen Qinlian decoction attenuated the elevated P10 protein (P<0.05). In small intestine tissue, compared with chow group, the protein levels of P10 in model group were increased. Both Gegen Qinlian decoction and glutamine group attenuated the elevated P10 protein (P<0.05). Conclusion The data suggested that Gegen Qinlian decoction could decrease the activation of NLRP3 both in liver and gut in experimental NASH model.
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