目的 观察二补助育改良方对胚泡着床障碍模型小鼠子宫内膜胞饮突、白血病抑制因子(leukemia inhibitory factor,LIF)、雌激素受体(estrogen receptor,ER)和孕激素受体(progesterone receptor,PR)表达的影响. 方法 建立小鼠胚泡着床障碍模型,将50只实验动物分为5组:空白组、模型组、西药组1(补佳乐组)、西药组2(阿司匹林组)、二补助育改良方组,每组10只.造模与给药同时进行,其后处死小鼠,扫描电镜观察胞饮突的表达,并采用免疫组化HBP法检测小鼠子宫内膜LIF、ER、PR的表达量. 结果 扫描电镜下,二补助育改良方组小鼠子宫内膜表面形态较规整,大量微绒毛及丰富胞饮突表达,细胞之间的间隙明显.免疫组化结果示,二补助育改良方组小鼠子宫内膜LIF、ER、PR表达较模型组明显增加(P<0.05);LIF表达低于补佳乐组(P<0.05),ER表达优于补佳乐组(P<0.05),PR表达与补佳乐组无明显差异(P>0.05);各指标与阿司匹林组比较无明显差异(P>0.05). 结论 二补助育改良方可能通过调节胚泡着床期子宫内膜胞饮突、LIF、ER和PR的表达,从而提高子宫内膜容受性,参与胚泡植入的黏附过程,改善着床障碍,创造良好的受孕环境,促进胚泡着床.%Objective To observe the effect of Erbu Zhuyu improving prescription on pinopodes, LIF, ER and PR expression in endometrium of mice model with dysfunctional embryo implantation.Methods 50 experimental animals were used to establish model of dysfunctional embryo implantation, and the mice were divided into five groups (10 in each group): blank group, model group, western medicine treated group 1 (progynova group), western medicine treated group 2 (aspirin group), Erbu Zhuyu improving prescription group.Pinopodes in uterine endometrium were examined by scanning electron microscopy.LIF, ER and PR expressions were detected by immunohistochemistry.Results Scanning electron microscopy showed that the endometrium of Erbu Zhuyu improving prescription group was more regular and the expression of pinopodes were abundant, and the space between the cells was obvious.Immunohistochemical results showed LIF, ER and PR expression of Erbu Zhuyu improving prescription group were obviously higher than model group (P<0.05).Compared with the progynova group, LIF level of Erbu Zhuyu improving prescription was lower (P<0.05) while ER level was higher, and PR level was no obvious difference(P>0.05), all above index of Erbu Zhuyu improving prescription was equal to the aspirin group.Conclusion Erbu Zhuyu improving prescription might regulate the expression of pinopodesand, LIF, ER and PR expression of blastocyst implantation period, improve the endometrial receptivity and contribute to the adhesion of embryo implantation, and to improve and implantation dysfunction, to create a good environment to promote pregnancy and embryo implantation.
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