以长江2号多花黑麦草种子为材料,设置5个NaCl浓度梯度(0.1%,0.2%,0.3%,0.4%,0.5%),通过测定发芽率、发芽势、幼苗生长速度及幼苗的SOD活性、MDA含量,分析盐胁迫对长江2号多花黑麦草种子发育及幼苗生长的影响.结果表明:种子的发芽率和发芽势,幼苗的株高和生长速度均受NaCl浓度影响.浓度大于0.1%时显著降低了种子发芽势,盐浓度大于0.2%时显著降低了种子发芽率.含盐量≤0.3%时显著降低了幼苗的相对生长速率.盐胁迫对幼苗鲜重和干重有显著影响,对幼苗含水量影响较小.0.1%NaCl处理下幼苗叶中SOD活性和CK差异显著(P<0.05),NaCl浓度大于0.2%时,叶中SOD活性和MDA含量逐步上升,0.5%NaCl胁迫下幼苗全部死亡.长江2号多花黑麦草的耐盐性较差,适宜在含盐量小于0.1%的土壤上种植和推广.%Germination rate,germination energy,seedling growth speed,SOD activity and MDA content of seedlings were studied under different NaCl concentrations (0.0%,0.1%,0.2%,0.3%,0.4% and 0.5%) with italian ryegrass(No.2 Changjiang)an indicator crop.The results showed that germination rate,germination en-ergy,height and growth speed of seedlings could be affected by NaCl concentrations.Germination rate signifi-cantly decreased as the NaCl concentration above 0.2% and germination energy significantly decreased as the NaCl concentration above 0.1%.The relative growth rate of seedlings significantly decreased as the NaCl con-centration above 0.3%.Effects of NaCl concentrations on fresh weight and dry weight of seedlings were signifi-cant,but that on water content of seedlings was not significant.SOD activity and MDA content under NaCl con-centration of 0.1% were significantly lower than those under the control (0.0%).SOD activity and MDA con-tent significantly increased as the NaCl concentration above 0.2%.Large amount of seedlings died under NaCl concentration of 0.5%.Italian ryegrass has a low salt tolerance,and was only suitable for production under NaCl concentration below 0.1%.
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