Objective To establish a method of di-allele-specific-amplification with artificially modified primers combined with SYBR Green I real-time PCR for gene polymorphisms analysis of GSTP1 codon 105, and to investigate hereditary character of glutathione S-transferase P1 (GSTP1) codon 105 among Guangxi Baise population by this method.Methods Gene polymorphisms of GSTP1 codon 105 were detected by di-allele-specific-amplification with artificially modified primers combined with SYBR Green I real-time PCR in 211 healthy Guangxi Baise people.The genotype frequency of GSTP1 codon 105 in Guangxi population was compared with other groups in China, other countries and regions.Results The GSTP1 Ile105Val genotypes detected by di-allele-specific-amplification with artificially modified primers combined with SYBR Green I real-time PCR were consisted with those detected by sequencing analysis of PCR products.The frequency of allele A in GSTP1 gene Ile105Val was both high in female and male populations (79.7% and 84.5%, respectively).The prevalence rates of GSTP1 Ile105Val genotypes AA, AG and GG were 62.2%, 35.1%, and 2.7% in Guangxi Baise female population, and were 71.0%, 27.0% and 2.0%, respectively, in males.There was no significant difference between female and male populations in allele or genotype frequency of GSTP1 Ile105Val among Guangxi Baise population.The distribution frequency of genotype in Guangxi Baise population had no significant difference with Liaoning, Ningxia Hui Ethnicity, India or Russian population, but significantly differed with other groups in Xinjiang Uighur, Mongol nationalities, Korean nationality, and Caucasian women (P<0.01).Conclusion Di-allele-specific-amplification with artificially modified primers combined with SYBR Green I real-time PCR is an easy, rapid, and accurate method for detection of gene polymorphisms of GSTP1.Gene polymorphism of GSTP1 in codon 105 has no gender difference, and is different from those in other regions of China and other countries.%目的 建立人工修饰双等位基因特异性引物结合SYBR Green I荧光PCR法检测GSTP1第105个密码子基因多态性的方法,并利用该法研究GSTP1 第105个密码子在广西百色市人群遗传特征,为进一步研究GSTP1基因多态性与疾病的发生易感性研究奠定基础.方法 采用人工修饰双等位基因特异性引物结合SYBR Green I荧光PCR法技术对211例广西百色市健康成人进行GSTP1 Ile105Val基因分型,并分析是否存在性别差异及与国内外其他人群分布频率差异.结果 人工修饰双等位基因特异性引物结合SYBR Green I荧光PCR法GSTP1基因型检测结果与PCR产物测序结果一致,用此法检测出广西百色市女性和男性人群GSTP1 Ile105Val均以A等位基因为主,分别为79.7%和84.5%;AA野生纯合子型、AG杂合子型和GG突变纯合型在女性人群中分别为62.2%、35.1%和2.7%,男性人群则为71.0%、27.0%和2.0%.经统计学分析,广西GSTP1Ile105Val等位基因和基因型分布频率无性别差异,与辽宁、回族、印度、俄罗斯族等人群相应位点基因型分布频率无差异,但与国内维族、朝鲜族、蒙古族、高加索女性相比差异有统计学意义(P<0.01).结论 人工修饰双等位基因特异性引物结合SYBR Green I荧光PCR法是一种快捷、准确的基因多态性检测方法.广西人群GSTP1基因 Ile105Val呈多态性分布,基因型分布频率无性别差异,与其他部分种族存在差异.
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