目的 探索艾灸对类风湿关节炎( RA)大鼠肿瘤坏死因子-α( TNF-α)昼夜节律的调控机制.方法 将144只SD大鼠随机分为空白组、模型组、艾灸组、假摘组、摘除组、摘灸组,各组分4个时间点(分别为0、6、12、18点) ,每个时间点6只大鼠. 各组大鼠实验全程给予12:12的光暗周期限制,除空白组外其余各组采用右后足足垫部皮内注入福氏完全佐剂建立RA模型. 造模后对摘除组、摘灸组大鼠作摘除双侧肾上腺预处理. 艾灸组、摘灸组大鼠在上午7:00—9:00进行单侧麦粒灸肾俞、足三里治疗(双侧交替治疗) ,其他组大鼠同法固定,但不艾灸治疗. 各组大鼠在造模前、造模后、治疗后分别进行体重及足容积测定. 治疗结束后分别在各个时间点处死大鼠,取血,用ELISA法检测相关指标. 结果 与空白组比较,模型组大鼠足肿胀度明显升高(P<0.05),TNF-α保持昼夜节律性(P<0.05),但峰相位有后移趋势,振幅有升高趋势,中值明显升高(P<0.05);与模型组比较,艾灸组大鼠足肿胀度明显降低(P<0.05),TNF-α存在昼夜节律性(P<0.05),峰相位有前移趋势,振幅有降低趋势,中值明显降低(P<0.05);与艾灸组比较,摘灸组大鼠足肿胀度明显升高(P<0.05),TNF-α昼夜节律消失,峰相位接近,振幅有降低趋势,中值明显升高(P<0.05). 结论 实验性RA大鼠血浆TNF-α具有明显的病理性昼夜节律特征;在下丘脑-垂体-肾上腺轴完整的条件下,艾灸可能通过调整实验性RA大鼠TNF-α的昼夜节律,从而发挥控制炎症的作用.%Objective To investigate the mechanism of of moxibustion on biological rhythm of TNF -αin treat-ment of rats with rheumatoid arthritis (RA).Methods One hundred and forty -four health Sprague-Dawley (SD) rats were randomly divided into normal group , RA model group, moxibustion treatment group , sham group, adrenalectomy group, adrenalectomy and moxibustion treatment ( AM) group.The RA model was established by footpad injection of Fre-und′s complete adjuvant (FCA).The 12:12 light-dark circles were given to all subjects .Adrenalectomized pretreatment was performed in adrenalectomy group and AM group .Moxibustion was performed on Shenshu ( BL23 ) and Zusanli (ST36) points in moxibustion group and AM group at the set time .The foot volumes and weights were recorded before and after modeling and after treatment .The plasma TNF -αlevels were assessed at 0, 6, 12, and 18 o′clock.Results Comparing with control group , significant increased foot volume was recorded in model group , with maintained TNF -αcircadian rhythm.The peak phase was postponed , with significant increase amplitude and median (P<0.05).Comparing with model group , the foot volume was significantly reduced in moxibustion group , with maintained TNF -αcircadian rhythm.The peak phase was anticipated, with significant reduction in amplitude and median (P<0.05).Comparing with moxibustion group , the foot volume was significantly increased in AM group , with loss of TNF-αcircadian rhythm .The peak phase was anticipated , with significant reduction in amplitude and increase in median ( P<0.05 ) .Conclusion Experimental RA rats have circadian rhythm of TNF -α.HPA axis plays an important role in adjusting the rhythm in rats with RA, via which the anti -inflammatory effect was produced by moxibustion treatment .
展开▼
