目的 了解广东地区HIV/HCV共感染患者和单纯HIV感染患者的感染途径及HIV-1病毒基因亚型分布特征,为艾滋病的治疗与预防提供实验室数据.方法 采用巢式RT-PCR对广东地区51例HIV/HCV共感染患者和48例单纯HIV感染患者HIV-1 ENV基因c2v3区域及GAG基因p17区域进行扩增,PCR产物测序后所获得序列进行HIV-1病毒基因亚型分析.结果 HIV/HCV共感染患者主要感染途径为静脉注射毒品,约占82％,其HIV-1有4种基因亚型,不同亚型以及比例分别为CRF01_AE (54.9％)、CRF07_BC (33.3％)、CRF08_BC(4％)和B '型(7.8％),CRF01_AE亚型为经静脉注射毒品感染的HIV/HCV共感染患者的主要H1V-1基因亚型,约占54.7％:单纯HIV感染患者主要感染途径为性,约占77％,其HIV-1有5种基因亚型,不同亚型及比例分剐为CRF01 _AE (77.1％)、CRF07_BC(10.4％)、CRF08_BC (6.2％)、B'型(4.2％)和B型(2.1％),CRF01_AE亚型为经性途径感染的单纯HIV感染患者的主要HIV-1基因亚型,约占78.4％.结论 广东省HIV/HCV共感染患者和单纯HIV感染患者中流行的HIV-1病毒基因亚型呈现多样性,尽管HIV/HCV共感染患者的主要感染途径与单纯HIV感染患者的不同,但HIV-1病毒主要基因亚型相同.%Objective To investigate the transmission routes and subtypes distribution of HIV in HIV/HCV co-infected and HIV mono-infected individuals in Guangdong Province.Methods Reverse transcription nested (RT-PCR) was performed to amplify the HIV-1 ENV c2v3 and GAG p17 gene regions from 51 HIV/HCV co-infected and 48HIV mono-infected individuals in Guangdong province.The PCR products were sequenced for HIV subtyping.Genetic analysis was done by MEGA4 software.Results Injection drug use (IDU,82％) was the main route of the transmission of HIV/HCV co-infection.The HIV subtype CRF01_AE (54.9％),CRF07_BC (33.3％),CRF08_BC (4％) and B'(7.8％) were identified; among which CRFO1 AE (54.7％) was proved the predominant one in HIV/HCV co-infection through IDU.The sexual contact (77％) was the main route of the transmission of HIV mono-infection.The HIV subtype CRF01_AE (77.1％),CRF07_BC (10.4％),CRF08_BC (6.2％),B' (4.2％) and B (2.1％) were identified; among which CRF01_AE (78.4％) was proved the predominant one in HIV mono-infection through sexual contact.Conclusion Sufficient diversity of HIV subtype in HIV/HCV co-infection or HIV mono-infection in Guangdong province is revealed.Although the major transmission routes of HIV/HCV co-infection and HIV mono-infection are differed,the major HIV subtype is identical.
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