Objective To investigate the expression of interleukin - 6 ( IL - 6 ) and interleukin - 18 ( IL - 18 ) mRNA in villi of patients with recurrent spontaneous abortion ( RSA ) and to investigate the pathogenesis of RSA. Methods Real - time quantitative reverse transcription - polymerase chain reaction was used to quantify the expression of IL - 6 mRNA and IL - 18 mRNA in villi of 63 patients with RSA ( RSA group ) and 50 normal early pregnant women( normal group ). The correlation between IL -6 and IL - 18 was analyzed. Results IL -6 mRNA was significantly lower, while IL - 18 mRNA was significantly higher, in RSA group than that in normal group. Meanwhile, there was significantly negative correlation between the IL - 6 and IL - 18 in RSA group ( r = - 0. 49, P < 0. 01 ). Although there was no significant correlation between the number of abortion and IL -6 mRNA, IL - 18 mRNA was significantly elevated with the increase of number of abortion ( P <0. 05 ). Conclusion The down - regulated IL - 6 or up - regulated IL - 18 can lead to RSA, as IL - 18 is furthermore positively correlated with the number of abortion.%目的 研究白细胞介素-6(IL-6)和白细胞介素-18(IL-18)在复发性流产(RSA)患者绒毛组织中的表达,探讨RSA的发病机制.方法 采用实时荧光定量逆转录聚合酶链反应技术,检测63例RSA妇女(RSA组)及50例正常早孕妇女(正常组)绒毛组织中IL-6 mRNA和IL-18 mRNA的表达水平,并将两者进行相关性分析.结果 RSA组绒毛组织中IL-6 mRNA的表达水平显著低于正常组,IL-18 mRNA水平显著高于正常组,相关分析显示,RSA组IL-6与 IL-18 mRNA两者之间表达水平呈负相关(r=-0.49,P＜0.01).IL-6 mRNA的表达水平与RSA患者流产次数无关,而IL-18 mRNA表达水平随着流产次数增多而增加,差异有统计学意义(P＜0.05).结论 正常妊娠较RSA患者绒毛组织IL-6水平高,当IL-6 表达降低或IL-18升高时,可能导致RSA,其中IL-18水平与流产次数呈正相关.
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