Objective To evaluate the correlation between the polymorphism of CYP2J2*7 and coronary heart disease ( CHD ) in Chinese Han population. Methods The genotype was analyzed with polymerase chain reaction - restriction fragment length polymorphlism ( PCR - RFLP ) in CHD group ( n = 347 ) and control group ( n = 355 ). Direct sequencing was applied in random samples for genotype confirmation. Results ( 1 ) The frequencies of GT/TT were 9. 51％ and 5. 07％ in CHD group and control group, respectively, with significant difference in distribution ( P = 0. 02, OR =1. 88 , 95％ CI: 1. 08 ～ 3. 27 ). ( 2 ) Although no significant difference was revealed in GT/TT distribution in male population between the two groups ( P = 0. 18 ), significant difference was revealed in females ( P = 0. 04 ). Conclusion There is significant correlation between CYP2J2 *7 and CHD in the Chinese Han female population.%目的 研究CYP2J2 *7(即G-50T)与中国汉族人冠心病的相关性.方法 采用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)法对703例(347例冠心病患者+356例对照者)无血缘关系的研究对象进行基因分型并随机抽取部分样本采用直接测序的方法验证基因型.结果 (1)GT/TT基因型在冠心病组和对照组的分布频率分别为9.51%和5.07%,两组比较差异有统计学意义(P=0.02,OR=1.88,95%CI:1.08～3.27);(2)GT/TT基因型在男性冠心病病例与男性对照组中的分布频率差异无统计学意义(P=0.18),在女性冠心病病例与女性对照组中的分布频率差异有统计学意义(P=0.04).结论 CYP2J2 *7突变与中国汉族人冠心病的发生具有相关性,这种相关性主要表现在女性人群中.
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