[Objective]The feasibility of producing acetone-butanol-ethanol(ABE)from sweet potato as raw material was studied.[Methods]ABE fermentation was carried out with Clostridium acetobutylicum J-3-3 which was isolated and identified in this study.The optimum fermentation process was determined by optimizing the fermentation medium and the fermenta-tion conditions.Fermentation products were determined by gas chromatography internal stand-ard method.[Results]The optimum medium composition:The initial concentration of sugar in sweet potato liquor was 100 g/L,soybean meal was 8 g/L,KH2PO4was 0.8 g/L,FeSO4·7H2O was 0.06 g/L,and the initial pH was 7.5.The optimum fermentation conditions were as follows:Temperature 37℃,initial pH(pH 5.8),seedling age 22 h,and inoculation a-mount 10％.Under this fermentation condition,the solvent yield reached the maximum after 60 h of fermentation.The yields of acetone,ethanol and butanol were 6.98 g/L,1.16 g/L and 15.34 g/L,respectively.The total solvent was 23.48 g/L.[Conclusion]The research result demonstrated that it was feasible to perform the ABE fermentation with sweet potato as raw material,which provides a new idea of broadening the source of raw material for producing ace-tone-butanol-ethanol from fermentation and deep processing of sweet potato.%[目的]研究以甘薯为原料发酵生产丙酮-丁醇-乙醇(ABE)的可行性.[方法]以本研究分离鉴定的丙酮丁醇梭菌(Clostridium acetobutylicum)J-3-3 为生产菌株,通过发酵培养基和发酵条件的优化,确定最佳的发酵工艺条件.发酵产物采用气相色谱的内标法测定.[结果]最佳发酵培养基组成:甘薯液化液初糖浓度为 100 g/L,豆粕 8 g/L,KH2PO40.8 g/L,FeSO4·7H2O 0.06 g/L.最佳发酵条件:温度 37℃,初始 pH 自然(pH 值为5.8),种龄22 h,接种量10％.在此发酵工艺条件下发酵60 h,溶剂产量达到最大,丙酮、乙醇和丁醇的产量分别为 6.98 g/L、1.16 g/L和 15.34 g/L,总溶剂 23.48 g/L.[结论]研究结果表明,以甘薯为原料发酵生产丙酮-丁醇-乙醇是可行的,这为拓宽发酵法生产丙酮-丁醇-乙醇的原料来源以及甘薯的深加工提供了新的思路.
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