To investigate the inhibitory effect of extracts from Helleborus thibetanus on cell proliferation and expres-sion of COX-2 mRNA,the inhibitory effects of the total ethanol extract (TKZ1),n-butanol fraction (TKZ2),and ethyl acetate fraction (TKZ3)on DU145,PC3,HeLa,HT-29 and HepG2 tumor cell lines were examined by MTT assay,and the COX-2 mRNA expression levels were analyzed by quantitative PCR.The results showed that TKZ1, TKZ2 and TKZ3 could significantly inhibit the proliferation of tumor cell lines and suppress the expression of COX-2 on the mRNA level in a dose-dependent manner compared with the control.All three samples from Helleborus thi-betanus (TKZ)could significantly inhibit the proliferation of tumor cells in vitro,and the mechanism might be related to the inhibition of the COX-2 mRNA expression in tumor cells.%研究不同铁筷子提取物对肿瘤细胞增殖及 COX-2 mRNA 表达的抑制作用。以铁筷子醇总提取物(TKZ1)、正丁醇萃取部位(TKZ2)、乙酸乙酯萃取部位(TKZ3)分别作用于 DU145、PC3、HeLa、HT-29、HepG2等肿瘤细胞，应用噻唑蓝实验(MTT 法)计算其对细胞增殖的抑制作用，应用荧光定量 PCR 技术检测TKZ1、TKZ2、TKZ3处理后的各肿瘤细胞中 COX-2 mRNA 的表达情况。结果表明：TKZ1、TKZ2、TKZ3均能显著抑制多种肿瘤细胞的增殖，与阴性对照组比较，其可以在 mRNA 水平上抑制 COX-2的表达，且呈明显的量效关系。说明铁筷子提取物对体外肿瘤细胞的增殖具有显著的抑制作用，其抗瘤机制可能与抑制肿瘤细胞中 COX-2 mRNA 的表达有关。
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