The residual amount of emamectin benzoate and imidacloprid in tomato and soil were determined by HPLC-UV to establish a rapid analysis method for determining residual amount of Emamectin benzoate and Imidacloprid in tomato and soil simultaneously.The results showed that when the adding amount of the mixed sample of emamectin benzoate and imidacloprid is 0.05 ~ 1.0 mg/kg,the added recovery of emamectin benzoate in tomato and soil is 82.54% ~ 86.60% with 0.96% ~ 3.30%relative deviation and 82.50%~96.21% with 0.79%~3.93% relative deviation,and the added recovery of imidacloprid in tomato and soil is 83.25% ~ 94.20% with 0.71% ~ 1.78% relative deviation and 90.52%~98.39% with 0.80%~3.24% relative deviation respectively; which indicates that the determinining method with good accuracy and precision can meet the demand of pesticide residues analysis and be suitably used in residual determination of emamectin benzoate and imidacloprid in tomato and soil. The residual amount of emamectin benzoate and imidacloprid in tomato and soil 5d after spraying determined by the method is 0.03~0.05 mg/kgand 0.05 ~ 0.10 mg/kg,and 0.03 ~ 0.05 mg/kg and 0.04~0.08 mg/kg respectively.The residual amounts of emamectin benzoate and imidacloprid in tomato and soil do not exceed national maximum residual limit in tomato.%为建立同时检测番茄及其土壤中甲维盐和吡虫啉残留的快速分析方法，采用丙酮/水（1∶1， V/V）提取样品，经硅胶和活性炭混合物层析柱净化，HPLC-UV 检测，对甲维盐和吡虫啉在番茄及其土壤中的残留进行了检测。结果表明：1）甲维盐和吡虫啉的混合标样添加量为0．05～1．0 mg/kg 时，甲维盐在番茄和土壤中的添加回收率分别为82．54％～86．60％和82．50％～96．21％，相对偏差分别为0．96％～3．30％和0．79％～3．93％；吡虫啉在番茄和土壤中的添加回收率分别为83．25％～94．20％和90．52％～98．39％，相对偏差分别为0．71％～1．78％和0．80％～3．24％，说明该方法的准确度和精密度均符合农药残留分析的要求，适合番茄及其土壤中甲维盐和吡虫啉的残留量检测。2）采用该方法对2013年番茄及其土壤中甲维盐、吡虫啉的残留状况进行检测表明，施药5 d 后的番茄甲维盐残留量为0．03～0．05 mg/kg，吡虫啉残留量为0．05～0．10 mg/kg；施药5 d 后，土壤中甲维盐的残留量为0．03～0．05 mg/kg，吡虫啉的残留量为0．04～0．08 mg/kg。两种农药在番茄和土壤中的残留量均未超过我国在番茄中最高残留限量标准的规定。
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