目的 探讨脑钠尿肽(BNP)基因rs198389 位点单核苷酸多态性(SNP)与冠心病(CHD)的关系.方法 取病例-对照方法,收集冠心病和健康者各961 例,应用Real-time qPCR (TaqMan 探针)技术进行基因分型,分析BNP 基因rs198389 位点SNP 与CHD危险性的关系.结果 CHD组和对照组比较,AA和GG基因型、A 等位基因和G 等位基因分布差异有统计学意义(P<0.05);GG 基因型和G 等位基因频率在病例组(3.6%和16.7%)明显高于对照组(2.1%和14.3%).与A 等位基因比较,G 等位基因使CHD 危险性显著增加(OR=1.20,95%CI=1.01~1.43,P=0.04).GG基因型者CHD危险性增加(P=0.04),但在调整年龄、性别、吸烟、饮酒、体重指数、血甘油三脂、高血压史、糖尿病史和CHD家族史等变量后结果差异无统计学意义(P=0.12).结论 BNP基因位点rs198389 的G等位基因使CHD危险性显著增加;BNP基因rs198389 位点SNP可能与CHD易感性相关,但不是其独立危险因素.%Objective To investigate the association between single nucleotide polymorphism rsl98389 in-brain natriuretic peptide (BNP) gene and coronary heart disease (CHD). Methods Patients with CHD (the CHD group, n=961) and 961 healthy subjects (n=961, the control group) were enrolled in this study. The BNP rsl98389 polymorphism was genotyped by real-time quantitative polymerase chain reaction (Real-time qPCR). The association between BNP rsl 98389 polymorphism and CHD was analyzed by SPSS10.0 software. Results There was statistically significant difference in the distribution of AA and GG genotypes between the CHD group and the control group (P< 0.05). The frequencies of GG genotype and G allele were significantly higher in CHD group (3.6% and 16.7%) than in the control group (2.1% and 14.3%). G allele carries significantly higher risk of CHD than A allele (P=0.04). GG genotype presented a significantly higher risk of CHD (P=0.04), but this risk was not more significant when adjusted for CHD risk factors by unconditional Logistic regression analysis (P=0.12). Conclusion The G allele of BNP gene rsl98389 site significantly increased the risk of CHD. The results suggest that BNP gene rsl98389 may be a susceptibility gene of CHD, but it is not independent risk factor.
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