目的：观察消癥散瘀方对子宫内膜异位症造模后大鼠MAPK信号通路表达的影响，探讨其治疗内异症的作用机理。方法将48只SD大鼠随机分为正常对照组、内异症模型组、消癥散瘀方组、丹那唑组各12只，采用免疫组化检测各组大鼠异位内膜C-SRC、EFR、ERK的蛋白表达。结果与正常对照组比较，模型组异位内膜C-SRC、EFR、ERK蛋白表达明显升高（均P＜0．01）；与模型组比较，消癥散瘀方组异位内膜C-SRC、EFR、ERK蛋白表达明显降低（均P＜0．01）。结论消癥散瘀方能够降调子宫内膜异位症模型大鼠MAPK信号通路，从而降低子宫内膜细胞侵袭、转移、种植的能力。%Objective To observe the effects of Xiao-Zheng-San-Yu-Fang on MAPK of EM model and to investigate its mechanisminthetreatmentofEM.Method 48ratswererandomlydividedintoacontrolgroup,amodelgroup,aXiao-Zheng-San-Yu-Fang group and a Danazol group, each consisting 12 rats.The positive expressions of C-SRC、EFR、ERK protein in the focal tissue were tested with immunohistochical method.Findings Compared with the normal control group, the positive expressions of C-SRC, EFR, and ERK protein in the focal tissue as observed in the model group were remarkably higher ( P<0.01);Compared with the model group, the positive expressions of C-SRC, EFR, ERK protein in the focal tissue as observed in the Xiao-Zheng-San-Yu-Fang group were remarkably lower(P<0.01).Conclusion Xiao-Zheng-San-Yu-Fang can decrease the positive expressions of C-SRC, EFR, and ERK protein in the focal tissue.Therefore, they can inhibit the development of heterotopic membrane and thus keep the cells from developing an ability to invade, transpose, and implant.
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