Objective To observe the effect of Pien Tze Huan on the serum amount of TNF -α, ICAM-1 and VCAM-1 after MCAO operation.Methods Middle cerebral artery occlusion (MCAO) rat model was estab-lished by improved EZ Longa method .74 healthy mature male SD rats were randomly divided into model group , sham operation group, small-dose, mid-dose, high-dose Pien Tze Huan group, positive control group 1 (nimodipine group) and positive control group 2 ( Naodesheng group ) .Rats were pretreated for 3 days before modeling and ad-ministered for 4 days after modeling, then were sacrificed.The amount changes of ICAM -1, VCAM-1and TNF-αwere observed by methods of ELISA .Results The amount of ICAM -1, VCAM-1 and TNF-αin the model group increased obviously after the MCAO operation compared with that in sham operation group ( P<0.01).The ex-pression in each treatment group was lower than that in the model group (P<0.05 or P<0.01).Conclusion Pien Tze Huan can decrease the serum amount of ICAM -1, VCAM-1 and TNF-αso as to protect the nerve function .%目的：观察片仔癀对局灶性脑梗死大鼠炎性损伤相关因子肿瘤坏死因子-α（TNF-α）、细胞间黏附分子-1（ ICAM-1）及血管细胞黏附分子-1（ VCAM-1）含量的影响。方法将74只SD标准性大鼠随机分成7组，即假手术组10只，按照改良的EZ Longa法制备大脑中动脉栓塞大鼠模型64只，将手术后符合评分标准的大鼠分为模型组10只，片仔癀大剂量组10只、中剂量组12只、小剂量组10只，脑得生组和尼莫地平组各10只。造模前给药3 d，造模后给药4 d，处死取材，参照酶联免疫吸附法（ ELISA）检测ICAM-1、VCAM-1及TNF-α的含量。结果模型组血清ICAM-1、VCAM-1及TNF-α含量较假手术组升高（ P＜0．01）；各给药组较模型组均有不同程度的降低，其中片仔癀大剂量组、脑得生组及尼莫地平组血清ICAM-1、VCAM-1、TNF-α含量较模型组降低（ P＜0．01）。结论片仔癀胶囊可以降低局灶性脑梗死后大鼠ICAM-1、VCAM-1及TNF-α的含量，对于神经功能具有保护作用。
展开▼
