为探讨刺槐种子活力丧失与种子内部有毒物质积累的关系，利用1963、1979和2010年的刺槐种子的不同浓度甲醇漫提液处理白菜种子，研究其对白菜种子发芽及幼苗生长的影响。结果表明：不同黔藏时间的刺槐种子甲醇浸提液（10％、20％、30％和40％）对白菜（Brassica campestris L．ssp．chinensis）种子发芽率和活力指数以及幼苗的苗高和根生长均有抑制作用，贮藏时闻越长抑制作用越明显。用40％漫提液处理，白菜种子不能萌发，1963年采集的刺槐种子30％漫提液处理白菜种子也不能萌发；随着削槐种子粒藏时闻的延长和漫提液浓度的增加，对白菜幼苗的苗高和根长的生长抑制性也在增加，且根长受到的抑制性更明显。说明刺槐种子在贮藏过程中有毒物质在逐渐积累。%In order to study the relationship between the loss of the seed vigor and accumulation of the seed toxic substances, methanol extract from Robinia pseudoacacia L. seeds （ stored in 1963,1979,2010 respectively） in different concentration were used to deal with cabbage（Brassica campestris L. ssp. chinensis）seeds. The results showed that the methanol extract （ 10 %, 20%,30 % and 40 %） of Robinia pseudoacacia L. seeds for different storage time all posed a inhibitory action on the germination rate, vigor index of seed（SVI）, height and root growth of cabbage seedlings, with the longer storage time,the more obvious the inhibitory action. The cabbage seed could not germinate with 40% methanol extracts, neither was the 30% methanol extracts of Robinia pseudoacacia L. seeds collected in 1963 l With the storage time and methanol extract concentration increased, the more obvious the inhibitory to the seedling height and root growth. It was concluded that the toxic substances were accumulated gradually during the storage.
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