T4 DNA连接酶（T4Lig）在基因工程中有重要作用，科研人员对该工具酶性质了解不全面易导致平端DNA连接效率很低、甚至失败。所以对T4Lig性质功能的全面认识有助于基因重组，特别是平端DNA连接中的应用。为了提高平端DNA连接效率，以及进一步阐明平端连接机理，本文综述了T4Lig酶分子大小、保真性、切口DNA连接机理、平端DNA连接端口检测、酶分子性质理性改造等研究进展，并对平端连接中有待解决的几个问题进行了展望。%T4 DNA ligase(T4Lig)is important in gene recombination. Because of wide usage and being provided by company,its property is not thoroughly understood,which leads to low efficiency,sometimes even failure,in blunt-end DNA ligation. In order to recombine gene efficiently in blunt end DNA ligation and to further elucidate ligation mechanism of blunt-end DNA,the paper summarized such properties as enzyme size,fidelity,ligation mechanism of nick DNA,assay methods of nick-sealing,and rational engineering of enzyme properties. Some unsolved problems in blunt end DNA ligation were also provided. Therefore,to understand thoroughly T4Lig properties is useful for genetic recombination in vitro.
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