探讨建立一种亚硝酸钠氧化法测定血清总胆红素的新方法.采用亚硝酸钠氧化法测定血清总胆红素反应前后吸光度的变化，与标准品相比较，求得标本中总胆红素的浓度.精密度试验批内精密度（CV）值为1.38％；批间CV值为1.74％.线性范围可达792μmol/L .与其他相关试剂比较γ=0.9967.可用于检测临床标本.%A new sodium nitrite oxidation method for measuring serum total bilirubin was establised. Sodium nitrite oxidation method was applied for detecting serum total bilirubin(TBIL)concentrations according to the absorbance changes,and the results were analysed,compared with the standard controls. The intra-assay CV were 1.38%. The interassay CV were 1.74%. The linear ranges were up to 792μmol/L. The sodium nitrite method was well correlated with Vanadate method and Diazo method. It can be used in routine detections of serum total TBIL.
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