To establish the method for the content determination of Puerarin in Fufangdanxiong pills, HPLC was adopted. The separation was performed on Agilent TC-C18(4.6 mm×250 mm, 5μm), column with methanol-water (25:75) as mobile phase. The detection wavelength was 250 nm. The flow rate was 1.0 mL·min-1. Rrsult was shown that linear range of Puerarin was 0.076 2～1.524 mg(R2=0.999 9) , with an average recovery of 95.6%(RSD=1.51%, n=6). The method is simple, accurate and well-separated for the quality control of Fufangdanxiong pills.%用高效液相色谱法测定复方丹芎滴丸中葛根素的含量,采用高效液相色谱法,色谱柱为C18柱(4.6 mm×250 mm,5μm),甲醇-水(25:75)为流动相,流速为1.0 mL· min-1,检测波长:250 nm.结果表明,葛根素在0.076 2～1.524 mg范围内与峰面积呈良好的线性关系,R2=0.999 9,平均回收率95.6％,RSD=1.51％ (n=6).说明该测定方法稳定,重现性好,结果准确可靠,可用于复方丹芎滴丸的质量控制.
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