The effects of carbon source,nitrogen sources on fermentation of ε-polylysine biosynthesis in shake flasks by Streptomyces albus strain N31-69 were studied.The results showed that content of glucose,(NH4)2SO4,yeast extract powder in the optimum medium was 30,7,7 g/L,respectively.The fermentation yield of ε-polylysine could reach to 1.519 g/L,increased by 28.0％ compared with the original medium.Batch fermentation of ε-polylysine in 5 L bioreactor was carried out.Controlling pH at 4.3 after 72 h of fermentation and controlling glucose concentration at 10～15 g/L by fed-batch strategy,the fermentation yield of ε-polylysine reached up to 15.60 g/L after 168 h of fermentation.%采用白色链霉菌N31-69菌株发酵制备ε-聚赖氨酸,考察碳源、氮源等因素对ε-聚赖氨酸产量的影响,优化发酵培养基.结果表明,优化的培养基为葡萄糖、(NH4)2SO4和酵母浸出粉的浓度分别为30、7、7 g/L,ε-聚赖氨酸摇瓶发酵产量达1.519 g/L,比优化前的产量提高了28.0％.进一步对N31-69菌株在5L发酵罐内的发酵工艺进行优化,发现发酵48 h后采用流加补糖方式控制还原糖浓度为10～15g/L,从72h开始控制pH为4.3,发酵168 h后ε-聚赖氨酸的产量可达15.60g/L.
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