目的：：观察开心颗粒对Aβ诱导的AD大鼠模型认知障碍的改善作用,并探讨其作用机制。方法：采用海马齿状回( dentate gyrus ,DG)内β淀粉样蛋白( amyloid-βpeptide ,Aβ)注射的方法建立阿尔茨海默病(Alzheimer' s disease,AD)大鼠模型。 Morris水迷宫对各组大鼠的学习记忆能力进行评价。TUNEL法对各组大鼠脑海马区凋亡的神经元进行标记。酶联免疫法( ELISA )测定每组大鼠脑内乙酰胆碱(acetylcholine,ACh)含量。结果：模型组大鼠学习记忆等认知功能下降,而且脑内海马区标记出大量凋亡神经元。开心颗粒组大鼠的认知功能明显改善,海马区凋亡神经元明显减少,同时脑内ACh水平明显提高。结论：开心颗粒能过通过抑制Aβ毒性以及提高脑内乙酰胆碱含量改善AD大鼠的认知功能。%Objective:To observe the effect of Kai -Xin Granule ( KXG) on the Alzheimer ' s disease of ( AD) model rats.Methods:Rats were injected by Amyloid βpeptide ( Aβ) into hippocampus dentate gyrus ( DG) to build AD models;Morris water maze was used to examine the cognitive function;TUNEL Assay was used to examine the neurons apoptosis and ELISA Assay was used to determine the expression level of the protein of acetylcholine (ACh).Results:In AD model rats, the cognitive ability decreased and apoptotic neuron was la-beled in the hippocampus DG area and a significant improvement in cognitive function was observed in the ani -mals administrated by KXG .Moreover, following administration of KXG , the histological injury in hippocam-pus DG induced by Aβwas improved and the ACh level in the brain was increased .Conclusion: KXG im-proves cognitive impairment in AD rats by protecting the brain neurons from Aβinduced apoptosis and increas-ing the cholinergic nerve function .
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