Objective: To study the conditions and parameters of purifying total asiaticosides in Centella asiatica urban with AB - 8 macropere resin, such as the pH, density, flowrate, amount of the upper column lipuor, ethanol density and amount of the elution column etc. Method: HPLC was used to analyze the content asiaticoside and madecasmside in order to study the adsorption conditions and the effect of desorption. Result: The appropriate adsorption conditions were:concentration of extract 0. 2g·mL-1 ;pH6 ～7 ;flow rate 2BV·h-1. When the ethanol concentration was 50％ and the amount was 3BV,the effect of desorption was satisfactory. Conclusion:AB -8 macropere resin has a good effect on the adsorption and desorption of total asiaticosides in Centella asiatica urban.%目的:研究AB-8大孔树脂分离纯化积雪草总苷的工艺条件及技术参数.方法:用高效液相色谱法测定积雪草苷和羟基积雪草苷的含量,考察AB-8大孔树脂对积雪草总苷的吸附及解吸性能.结果:AB-8大孔树脂对积雪草总苷的适宜吸附条件为:药液质量浓度0.2g·mL-1(相当于生药材),pH值6～7,流速2BV·h-1(BV为柱床体积倍数),洗脱剂用3BV 50%乙醇.结论:AB-8大孔树脂吸附积雪草总苷的纯化方法可行,具有较好的应用前景.
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