Molecular characterization of three Hsp90 from Pieris and expression patterns in response to cold and thermal stress in summer and winter diapause of Pieris melete

Yue-Kun Wu; Chao Zou; Dao-Meng Fu; Wan-Na Zhang; Hai-Jun Xiao

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Molecular characterization of three Hsp90 from Pieris and expression patterns in response to cold and thermal stress in summer and winter diapause of Pieris melete

机译：Pieris melete夏季和冬季滞育过程中3种来自Pieris的Hsp90的分子鉴定及其表达模式对冷热胁迫的响应

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Heat shock proteins (Hsps) have been linked to stresses and winter diapause in insects,but whether they are components of summer diapause is still unknown.In this study,complementary DNAs of Hsp90 from Pieris melete,Pieris rapae and Pieris canidia named PmHsp90,PrHsp90 and PcHsp90,respectively,were cloned and sequenced.The deduced amino acid sequence consisted of 718 amino acid residues with a putative molecular mass of 82.6,82.6 and 82.7 kDa,respectively.The amino acid sequences contained all of the five conserved signature motifs in the Hsp90 family and a bHLH protein folding activity region.The differential expression pattern of PmHsp90 in response to summer diapause and winter diapause,which are related to heat/cold stress,was investigated.Cold stress induced Hsp90 up-regulation in summer and winter diapause pupae,but not in non-diapause individuals.Heat shock up-regulated PmHsp90 gradually with an increase in temperature ir summer diapause,and PmHsp90 was rapidly up-regulated in winter diapause.After 30 min heat shock at 39℃,substantial up-regulation of PmHsp90 transcript levels were observed both in summer and winter diapause.However,in non-diapause a relatively stable expression was found under different durations of 39℃ heat shock.Compared to the optimal treatment of 18℃ for diapause development,a high temperature acclimation of 31 ℃ induced PmHsp90 up-regulation in summer diapause,whereas a low temperature acclimation of 4℃ induced up-regulation in winter diapause.The current results indicate that Hsp90 may play an important role in response to heat/cold stress both in summer and winter diapause.

机译：热休克蛋白（Hsps）与昆虫的应激和冬季滞育有关，但它们是否是夏季滞育的组成仍是未知的。推导的氨基酸序列由718个氨基酸残基组成，推定的分子量分别为82.6、82.6和82.7 kDa。研究Hsp90家族和bHLH蛋白折叠活性区域，但不是在非顽固的个体中。热休克随着夏季滞育期温度的升高而逐渐上调PmHsp90，而PmHsp90在夏季持续升高。在39℃的热休克后30分钟，夏季和冬季的滞育期间均观察到PmHsp90转录水平的显着上调。但是，在非暂停状态下，在39℃的热休克的不同持续时间中，发现相对稳定的表达。与18℃的滞育发育最佳治疗相比，夏季滞育时高温适应31℃诱导PmHsp90上调，冬季滞育时低温适应4℃诱导上调。在夏季和冬季滞育期间，在应对热/冷应激方面可能起重要作用。

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《中国昆虫科学：英文版》 |2018年第2期|273-283|共11页
作者
Yue-Kun Wu; Chao Zou; Dao-Meng Fu; Wan-Na Zhang; Hai-Jun Xiao;
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Institute of Entomology, Jiangxi Agricultural University, Nanchang, China;

Institute of Entomology, Jiangxi Agricultural University, Nanchang, China;

Institute of Entomology, Jiangxi Agricultural University, Nanchang, China;

Institute of Entomology, Jiangxi Agricultural University, Nanchang, China;

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China;

Institute of Entomology, Jiangxi Agricultural University, Nanchang, China;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
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Molecular characterization of three Hsp90 from Pieris and expression patterns in response to cold and thermal stress in summer and winter diapause of Pieris melete

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