Sugar beet root maggot (SBRM,Tetanops myopaeformis von R(o)der) is a major but poorly understood insect pest of sugar beet (Beta vulgaris L.).The molecular mechanisms underlying plant defense responses are well documented,however,little information is available about complementary mechanisms for insect adaptive responses to overcome host resistance.To date,no studies have been published on SBRM gene expression profiling.Suppressive subtractive hybridization (SSH) generated more than 300 SBRM ESTs differentially expressed in the interaction of the pest with a moderately resistant (F 1016) and a susceptible (F1010) sugar beet line.Blast2GO v.3.2 search indicated that over 40％ of the differentially expressed genes had known functions,primarily driven by fruit fly D.melanogaster genes.Expression patterns of 18 selected EST clones were confirmed by RT-PCR analysis.Gene Ontology (GO) analysis predicted a dominance of metabolic and catalytic genes involved in the interaction of SBRM with its host.SBRM gencs functioning during development,regulation,cellular process,signaling and under stress conditions were annotated.SBRM genes that were common or unique in response to resistant or susceptible interactions with the host were identified and their possible roles in insect responses to the host are discussed.
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机译：甜菜根（SBRM，Tetanops myopaeformis von R（o）der）是甜菜（Beta vulgaris L.）的主要但知之甚少的害虫。植物防御反应的分子机制已有很好的文献记载，但是，有关信息很少目前尚无关于SBRM基因表达谱的研究。抑制性消减杂交（SSH）产生了300多个SBRM EST，在害虫与中度昆虫的相互作用中差异表达抗性（F 1016）和易感性（F1010）甜菜品系。Blast2GOv.3.2搜索表明，超过40％的差异表达基因具有已知功能，主要由果蝇D.melanogaster基因驱动。18种选择的EST的表达模式RT-PCR分析证实了这些克隆。基因本体论（GO）分析预测了SBRM与它的相互作用与代谢和催化基因有关。注释了在发育，调控，细胞过程，信号转导以及在胁迫条件下起作用的SBRM基因。鉴定了与宿主抗性或易感性相互作用共同或独特的SBRM基因，以及它们在昆虫对宿主的反应中的可能作用讨论。
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