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首页> 外文期刊>中国昆虫科学：英文版 >Identification of LmUAP1 as a 20-hydroxyecdysone response gene in the chitin biosynthesis pathway from the migratory locust, Locusta migratoria
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Identification of LmUAP1 as a 20-hydroxyecdysone response gene in the chitin biosynthesis pathway from the migratory locust, Locusta migratoria

机译：LmUAP1作为来自蝗虫的几丁质生物合成途径中的20-羟基蜕皮激素反应基因的鉴定

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摘要

In Locusta migratoria,we found that two chitin biosynthesis genes,UDP N-acetylglucosamine pyrophosphorylase gene LmUAP1 and chitin synthase gene LmCHS1,are expressed mainly in the intcgument and are responsible for cuticle formation.However,whether these genes are regulated by 20-hydroxyecdysone (20E) is still largely unclear.Here,we showed the developmental expression pattern of LmUAP1,LmCHS1 and the corresponding 20E titer during the last instar nymph stage of locust.RNA interference (RNAi) directed toward a common region of the two isoforms of LmEcR (LmEcRcom) reduced the expression level of LmUAP1,while there was no difference in the expression of LmCHS1.Meantime,injection of 20E in vivo induced the expression of LmUAP1 but not LmCHS1.Further,we found injection-based RNAi of LmEcRcom resulted in 100％ mortality.The locusts failed to molt with no apolysis,and maintained in the nymph stage until death.In conclusion,our preliminary results indicated that LmUAP1 in the chitin biosynthesis pathway is a 20E late-response gene and LmEcR plays an essential role in locust growth and development,which could be a good potential target for RNAi-based pest control.
机译：在南方蝗中，我们发现两个甲壳质生物合成基因UDP N-乙酰氨基葡糖焦磷酸化酶基因LmUAP1和甲壳质合酶基因LmCHS1主要在表皮中表达并负责角质层的形成。然而，这些基因是否受20-羟基蜕皮激素（ 20E）在很大程度上仍不清楚。在这里，我们显示了LmUAP1，LmCHS1的发育表达模式以及蝗虫最后一个若虫阶段的相应20E效价。RNA干扰（RNAi）指向LmEcR（ LmEcRcom）降低了LmUAP1的表达水平，而LmCHS1的表达没有差异。同时，体内注射20E诱导了LmUAP1的表达，但没有诱导LmCHS1的表达。蝗虫没有蜕皮而没有蜕皮，并且一直保持在若虫阶段直至死亡。总之，我们的初步结果表明，壳多糖生物合成中的LmUAP1该途径是一种20E后期反应基因，LmEcR在蝗虫的生长和发育中起着至关重要的作用，这可能是基于RNAi的害虫控制的良好潜在靶标。

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  • 来源
    《中国昆虫科学：英文版》 |2018年第2期|211-221|共11页
  • 作者

    Xiao-Jian Liu; Ya-Wen Sun; Da-Qi Li; Sheng Li; En-Bo Ma; Jian-Zhen Zhang;

  • 作者单位

    Research Institute of Applied Biology, Shanxi University, Taiyuan, China;

    Research Institute of Applied Biology, Shanxi University, Taiyuan, China;

    College of Life Science, Shanxi University, Taiyuan, China;

    Research Institute of Applied Biology, Shanxi University, Taiyuan, China;

    Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China;

    Research Institute of Applied Biology, Shanxi University, Taiyuan, China;

    Research Institute of Applied Biology, Shanxi University, Taiyuan, China;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
  • 原文格式 PDF
  • 正文语种 eng
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