Possibly similar genetic basis of resistance to Bacillus thuringiensis Cry1Ab protein in 3 resistant colonies of the sugarcane borer collected from Louisiana, USA

Fei Yang; Mao Chen; Anilkumar Gowda; David L.Kerns; Fangneng Huang

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Possibly similar genetic basis of resistance to Bacillus thuringiensis Cry1Ab protein in 3 resistant colonies of the sugarcane borer collected from Louisiana, USA

机译：在美国路易斯安那州收集的三个甘蔗蛀虫抗性菌落中，对苏云金芽孢杆菌Cry1Ab蛋白抗性的可能相似的遗传基础

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The sugarcane borer,Diatraea saccharalis (F.),is a major maize borer pest and a target of transgenic maize expressing Bacillus thuringiensis (Bt) proteins in South America and the mid-southern region of the United States.Evolution of resistance in target pest populations is a great threat to the long-term efficacy of Bt crops.In this study,we compared the genetic basis of resistance to CrylAb protein in 3 resistant colonies of sugarcane borer established from field populations in Louisiana,USA.Responses of larvae to the Cry lAb protein for the parental and 10 other cross colonies were assayed in a diet-incorporated bioassay.All 3 resistant colonies were highly resistant to the CrylAb protein with a resistance ratio of ＞ 5 5 5.6 fold.No maternal effect or sex linkage was evident for the resistance in the 3 colonies;and the resistance was functionally nonrecessive at the CrylAb concentrations of ＜ 3.16 μg/g,but it became recessive at ＞10 μg/g.In an interstrain complementation test for allelism,the F1 progeny from crosses between any 2 of the 3 resistant colonies exhibited the similar resistance levels as their parental colonies,indicating that the 3 colonies most likely shared a locus of CrylAb resistance.Results generated from this study should provide useful information in developing effective strategies for managing Bt resistance in the insect.

机译：甘蔗bore虫（Diatraea saccharalis（F.））是一种主要的玉米pest虫害，是南美和美国中南部地区表达苏云金芽孢杆菌（Bt）蛋白的转基因玉米的靶标。种群对Bt作物的长期功效构成了巨大威胁。在本研究中，我们比较了美国路易斯安那州田间种群建立的3个甘蔗蛀虫抗性菌落对CrylAb蛋白抗性的遗传基础。通过饮食结合的生物测定法检测了亲本和其他10个交叉菌落的Cry lAb蛋白。所有3个耐药菌落均对CrylAb蛋白具有高度耐药性，耐药率> 5 5 5.6倍，没有母体效应或性别联系在三个菌落中的抗性;在CrylAb浓度<3.16μg/ g时，抗性在功能上无隐性，但在＞ 10μg/ g时变为隐性。分子分裂症，来自3个抗性菌落中任意2个的杂交后代的F1表现出与其亲本菌落相似的抗性水平，表明3个菌落最有可能共享CrylAb抗性基因座。这项研究产生的结果应为开发抗性提供有用的信息。控制昆虫对Bt抗性的有效策略。

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《中国昆虫科学：英文版》 |2018年第2期|241-250|共10页
作者
Fei Yang; Mao Chen; Anilkumar Gowda; David L.Kerns; Fangneng Huang;
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作者单位

Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA;

Monsanto Company,Chesterfield, Monsanto, USA;

Monsanto Company,Chesterfield, Monsanto, USA;

Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA;

Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, Louisiana, USA;

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收录信息中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
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Possibly similar genetic basis of resistance to Bacillus thuringiensis Cry1Ab protein in 3 resistant colonies of the sugarcane borer collected from Louisiana, USA

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