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Prediction of effective RNA interference targets and pathway-related genes in lepidopteran insects by RNA sequencing analysis

机译：通过RNA测序分析预测鳞翅目昆虫中有效的RNA干扰靶标和途径相关基因

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摘要

When using RNA interference (RNAi) to study gene functions in Lepidopterainsects,we discovered that some genes could not be suppressed;instead,their expressionlevels could be up-regulated by double-stranded RNA (dsRNA).To predict which genescould be easily silenced,we treated the Asian corn borer (Ostriniafurnacalis) with dsGFP(green fluorescent protein) and dsMLP (muscle lim protein).A transcriptome sequenceanalysis was conducted using the cDNAs 6 h after treatment with dsRNA.The resultsindicated that 160 genes were up-regulated and 44 genes were down-regulated by the twodsRNAs.Then,50 co-up-regulated,25 co-down-regulated and 43 unaffected genes wereselected to determine their RNAi responses.All the 25 down-regulated genes were knockeddown by their corresponding dsRNA.However,several of the up-regulated and unaffectedgenes were up-regulated when treated with their corresponding dsRNAs instead of beingknocked down.The genes up-regulated by the dsGFP treatment may be involved in insectimmune responses or the RNAi pathway.When the immune-related genes were excluded,only seven genes were induced by dsGFP,including ago-2 and dicer-2.These results notonly provide a reference for efficient RNAi target predications,but also provide somepotential RNAi pathway-related genes for further study.
机译：当使用RNA干扰（RNAi）研究鳞翅目昆虫的基因功能时，我们发现某些基因不能被抑制;相反，它们的表达水平可以被双链RNA（dsRNA）上调。为了预测哪些基因可以很容易地沉默，我们用dsGFP（绿色荧光蛋白）和dsMLP（肌肉lim蛋白）处理了亚洲玉米bore（Ostriniafurnacalis）。用dsRNA处理6小时后，用cDNA进行了转录组序列分析。结果表明，上调了160个基因，其中44个两个dsRNA下调了这些基因，然后选择了50个共同上调的基因，25个共同下调的基因和43个未受影响的基因来确定其RNAi反应，而这25个下调的基因全部被其对应的dsRNA敲除了。 ，用相应的dsRNA处理而不是被敲除的几个上调基因和未受影响的基因上调。通过dsGFP处理上调的基因可能与昆虫相关。排除免疫相关基因后，dsGFP仅诱导了7个基因，包括ago-2和dicer-2。这些结果不仅为有效的RNAi靶标预测提供参考，还提供了潜在的RNAi。通路相关基因有待进一步研究。

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  • 来源
    《中国昆虫科学：英文版》 |2018年第3期|356-367|共12页
  • 作者

    Ruo-Bing Guan; Hai-Chao Li; Xue-Xia Miao;

  • 作者单位

    Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;

    Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;

    Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China;

  • 收录信息 中国科学引文数据库(CSCD);中国科技论文与引文数据库(CSTPCD);
  • 原文格式 PDF
  • 正文语种 eng
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