Molecular elements of pheromone detection in the female moth, Heliothis virescens

Monika Zielonka; Heinz Breer; Jürgen Krieger

摘要

Pheromones play pivotal roles in the reproductive behavior of moths,most prominently for the mate finding of male moths.Accordingly,the molecular basis for the detection of female-released pheromones by male moths has been studied in great detail.In contrast,little is known about how females can detect pheromone components released by themselves or by conspecifics.In this study,we assessed the antenna of female Heliothis virescens for elements of pheromone detection.In accordance with previous findings that female antennae respond to the sex pheromone component (Z)-9-tetradecenal,we identified olfactory sensory neurons that express its cognate receptor,the receptor type HR6.All HR6 cells coexpressed the “sensory neuron membrane protein 1” (SNMP1) and were associated with supporting cells expressing the pheromone-binding proteins PBP1 and PBP2.These features are reminiscent to male antennae and point to congruent mechanisms for pheromone detection in the two sexes.Further analysis of the SNMP1-expressing cells revealed a higher number in females compared to males.Moreover,in females,the SNMP1 neurons were arranged in clusters,which project their dendrites into a common sensillum,whereas in males there were only solitary SNMP 1-neurons and only 1 per sensillum.Not all SNMP1 positive cells in female antennae expressed HR6 but instead the putative pheromone receptors HR11 and HR18,respectively.Neurons expressing 1 of the 3 receptor types were assigned to different sensilla.Together the data indicate that on the antenna of females,sensory neurons in a subset of sensilla trichodea are equipped with molecular elements,which render them responsive to pheromones.

机译：信息素在蛾的繁殖行为中起着关键作用，最主要是在雄蛾的伴侣发现中。因此，对雄蛾检测雌性释放信息素的分子基础进行了详细研究。相反，已知的很少。关于女性如何检测自己或特定物种释放的信息素成分的方法。在这项研究中，我们评估了女性Heliothis virescens的触角，以进行信息素的检测。根据先前的发现，女性触角对性信息素成分（Z）有反应- 9-decenal，我们鉴定出表达其同源受体HR6受体的嗅觉感觉神经元。所有HR6细胞共表达“感觉神经元膜蛋白1”（SNMP1），并与表达信息素结合蛋白PBP1和PBP2的支持细胞相关。这些特征使人联想到男性触角，并指出了男女性别信息素检测的一致机制。与雄性相比，表达SNMP1的细胞在雌性中的数量要多于雄性。此外，在雌性中，SNMP1神经元成簇排列，将其树突投射到一个共同的感觉区，而在雄性中，只有孤独的SNMP 1-神经元，并且只有每个sensillum 1个。并不是女性触角中的所有SNMP1阳性细胞都表达HR6，而是假定的信息素受体HR11和HR18分别表达了3种受体类型中的1种的神经元被分配给了不同的感觉器。感性线粒体亚群中的感觉神经元配备了分子元素，使其对信息素有反应。

Molecular elements of pheromone detection in the female moth, Heliothis virescens

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